- A20087H (See other available formats)
- Other Names
- Nucleocapsid phosphoprotein, N, Nucleoprotein
- Mouse IgG2b, κ
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- Product Citations
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Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is a single stranded RNA virus that belongs in a family of viruses known as coronaviruses. SARS-CoV-2 infection, known as COVID-19, was declared a pandemic in 2020 and among other symptoms leads to respiratory infection, pulmonary failure which can be fatal. SARS-CoV-2 is structurally composed of 4 main proteins (e.g. spike glycoprotein, envelope glycoprotein, membrane glycoprotein and nucleocapsid protein) and several accessory proteins.
The SARS-CoV-2 Nucleocapsid (N) is an abundant protein, that binds to the viral RNA and helps stabilize it. It has been proposed that N protein forms bimolecular condensates that both regulate viral replication and modulate the host response to the virus following infection. N protein is a major antigen that the host immune response is directed to following SARS CoV-2 infection making it a promising candidate for vaccine development and drug therapy.
- Antibody Type
- Host Species
- SARS-CoV-2 Nucleocapsid recombinant protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
Direct ELISA, ICFC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For Direct ELISA application, a concentration of 3.7 ng/mL is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
SARS-CoV-2 is 90% homologous to SARS-CoV. Cross reactivity of anti-SARS-CoV-2 Nucleocapsid antibody (clone A20087H) with SARS-CoV has not been tested.
BioLegend offers two clones specific for SARS-CoV-2 nucleocapsid, clones A20087F and A20087H
- Both clones have been verified for western blot, with A20087F showing a higher affinity for SARS-CoV-2 nucleocapsid for this application
- Both clones have been verified for direct ELISA and displayed similar EC50 values for this application
- A20087H has been verified for ICFC, while A20087F is not recommended for this application
- SARS-CoV-2 is a 419 amino acid protein with an expected molecular weight of 46 kD
Viral nucleoprotein, host cell Golgi apparatus
- RNA binding, transcription regulation
- Antigen References
- Zeng W. et al. 2020. Biochem Biophys Res Commun 527(3):618-623.
- Yoshimoto FK. 2020. Protein J. 39(3):198-216.
- Cascarina SM. & Ross ED. 2020. The FACEB Journal. 34:9832-9842.
- Gene ID
- 43740575 View all products for this Gene ID
- View information about SARS-CoV-2 on UniProt.org
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