Purified anti-rat CD4 Antibody

Pricing & Availability
W3/25 (See other available formats)
Regulatory Status
Other Names
T4, L3T4, W3/25
Mouse IgG1, κ
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Product Citations
Lou rat splenocytes stained with purified W3/25, followed by anti-mouse IgG FITC
  • W3slash25_Pure_032808
    Lou rat splenocytes stained with purified W3/25, followed by anti-mouse IgG FITC
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201501 100 µg 100€
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CD4 is a 55 kD glycoprotein also known as T4. Rat CD4 is a member of the immunoglobulin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Rat thymocyte membrane glycoproteins
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, inhibition of IL-2 production3, inhibition of MBP-induced T cell activation in EAE transfer model3.

Application References

(PubMed link indicates BioLegend citation)
  1. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  2. Shioji K, et al. 2001. Circulation Res. 89:540. (IHC)
  3. Mannie MD, et al. 1993. J. Immunol. 151:7293.
  4. Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed
Product Citations
  1. Kurtz CC, et al. 2007. Dev Comp Immunol. 31:415. PubMed
  2. Cao S, et al. 2022. Theranostics. 12:6038. PubMed
  3. Harms AS, et al. 2017. Acta Neuropathol Commun. 0.267361111. PubMed
  4. Tang-Huau TL, et al. 2021. Viruses. 13: . PubMed
  5. Beetz O, et al. 2019. PLoS One. 14:e0220546. PubMed
AB_313931 (BioLegend Cat. No. 201501)

Antigen Details

Ig superfamily, 55 kD

Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells)

T cell co-receptor, T cell activation
MHC class II molecule
Cell Type
Macrophages, T cells, Thymocytes
Biology Area
Molecular Family
CD Molecules
Antigen References

1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609.
2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649.

Gene ID
24932 View all products for this Gene ID
View information about CD4 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • PerCP/Cyanine5.5 anti-rat CD4


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