Purified anti-rat CD11a Antibody

Pricing & Availability
Clone
WT.1 (See other available formats)
Regulatory Status
RUO
Other Names
LFA-1 α chain, αL Integrin, ITGAL, Ly-15, Ly-21
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
WT-1_PURE_CD11a_FC_080318
Rat splenocytes were stained with purified anti-rat CD11a (clone WT.1; filled histogram) or mouse IgG2a, κ isotype control (open histogram) followed by anti-mouse IgG PE.
  • WT-1_PURE_CD11a_FC_080318
    Rat splenocytes were stained with purified anti-rat CD11a (clone WT.1; filled histogram) or mouse IgG2a, κ isotype control (open histogram) followed by anti-mouse IgG PE.
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201902 100 µg 76,00€
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Description

CD11a is a 160-170 kD glycoprotein also known as αL integrin, or LFA-1 α chain. It is a member of the integrin family and forms a heterodimer with β2 integrin (CD18). The LFA-1 (CD11a/CD18) is broadly expressed on leukocytes and mediates intercellular adhesion and cellular activation. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are the ligands of LFA-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
PHA-stimulated rat splenocytes and rat thymic lymphoma FTL-43
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Block, IHC-F, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The WT.1 antibody recognizes both activated and unactivated LFA-1 and inhibits the binding of LFA-1 to ICAM-1. The WT.1 antibody has been shown to inhibit the mixed lymphocyte reaction, leukocyte infiltration, and graft rejection. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, immunoprecipitation3, in vivo and in vitro blocking of cellular adhesion3-6.

Application References

(PubMed link indicates BioLegend citation)
  1. Andrews FJ, et al. 1997. Dig. Dis. Sci. 42:326. (IHC)
  2. Springer TA. 1994. Cell 76:301. (IHC)
  3. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627. (IP, Block)
  4. Devine L, et al. 1996. Immunol. 88:456. (Block)
  5. Ohta Y, et al. 1998. Transplantation 65:1094. (Block)
  6. Watanabe T, et al. 1997. Am. J. Pathol. 150:971. (Block)
Product Citations
RRID
AB_2749874 (BioLegend Cat. No. 201902)

Antigen Details

Structure
160-170 kD glycoprotein
Distribution

Leukocytes including lymphocytes, monocytes/macrophages, and granulocytes.

Function
Mediates intercellular adhesion and cellular activation.
Interaction
CD18
Ligand/Receptor
CD54 (ICAM-1), CD102 (ICAM-2), CD50 (ICAM-3)
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627.
2. Tamatani T, et al. 1991. Eur. J. Immunol. 21:855.

Gene ID
308995 View all products for this Gene ID
UniProt
View information about CD11a on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08.03.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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