Purified anti-Raptor Antibody

Pricing & Availability
Clone
Poly6232 (See other available formats)
Other Names
Regulatory associated protein of mTOR, p150 target of rapamycin (TOR)-scaffold protein
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
poly6232_PURE_raptor_Antibody_1_WB_092116
Total cell lysates (15 µg protein) from NIH3T3 and Jurkat were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with anti-Raptor Antibody (Clone Poly6232). Proteins were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection. GAPDH Antibody was used as a loading control.
  • poly6232_PURE_raptor_Antibody_1_WB_092116
    Total cell lysates (15 µg protein) from NIH3T3 and Jurkat were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with anti-Raptor Antibody (Clone Poly6232). Proteins were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection. GAPDH Antibody was used as a loading control.
  • poly6232_PURE_raptor_Antibody_2_IHC_092116
    Formalin-fixed paraffin-embedded human kidney tissue was stained with Poly6232 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
Cat # Size Price Quantity Avail. Save
623202 200 µl (20 Western blots) 200€
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Description

Raptor, also known as regulatory associated protein of mTOR, is a scaffold protein of approximately 150 kD that is highly expressed in skeletal muscle. Raptor functions as an essential scaffold for mTOR-catalyzed phosphorylation of 4EBP1 critical for TOR activity in vivo. Raptor is involved in nutrient-stimulated signaling to the downstream effector RPSKB1 and has been shown to negatively regulate the FRAP1 kinase under conditions of nutrient deprivation. Raptor/FRAP1 interactions are critical for the coordination of cell size and cell growth under different environmental conditions. The Raptor protein has been shown to interact with mTOR, 4EBP1, RPS6KB1 and FRAP1. The Poly6232 antibody recognizes human Raptor and has been shown to be useful for Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Peptide-KLH
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

WB - Quality tested
IHC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:50 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

RRID
AB_2249473 (BioLegend Cat. No. 623202)

Antigen Details

Structure
mTOR scaffold protein, contains 7 WD repeats, 150 kD. Alternatively spliced variants have been reported.
Distribution

Highly expressed in skeletal muscle, expressed in lower amounts in brain, lung, small intestine, kidney, and placenta.

Function
Essential scaffold for mTOR-catalyzed phosphorylation of 4EBP1 critical for TOR activity in vivo. Has positive role in nutrient-stimulated signaling to the downstream effector RPSKB1. Raptor negatively regulates FRAP1 kinase under conditions of nutrient-d
Interaction
Binds to mTOR, 4EBP1 amd RPS6KB1; forms complex with FRAP1.
Biology Area
Cell Biology, Signal Transduction
Antigen References

1. Hara K, et al. 2002. Cell 110:177.
2. Yonezawa K, et al. 2004. Biochem. Biophys. Res. Commun. 313:437.
3. Kim DH, et al. 2002. Cell 110:163.
4. Oshiro N, et al. 2004. Genes Cells 9:359.

Gene ID
57521 View all products for this Gene ID
UniProt
View information about Raptor on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09.21.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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