- W18011A (See other available formats)
- Regulatory Status
- Other Names
- TERF2IP, TERF2 Interacting Protein, TERF2-interacting telomeric protein 1, TRF2-interacting telomeric protein 1, Repressor/activator protein 1 homolog, Dopamine receptor-interacting protein 5, DRIP5
- Rat IgG2b, κ
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- Product Citations
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Repressor/activator protein 1 (RAP1), also called telomeric repeat-binding factor 2-interacting protein 1 (TERF2IP), is one of six subunits of the shelterin complex, which regulates telomerase activity and protects telomeric DNA through the generation of t-loops. RAP1 forms a heterodimer with shelterin subunit TRF2 and regulates telomere length, recombination, and transcription. This complex also functions in the repression of homology-directed repair (HDR) and prevents telomere deletions. In the cytoplasm, RAP1 functions as an inhibitor kinase (IKK) adaptor and modulator of the nuclear factor- κB (NF-κB) pathway as well as an activator of NF-κB transcriptional activity. TERF2IP S205 phosphorylation has been implicated in the regulation of activation, apoptosis, and senescence of endothelial cells.Product Details
- Antibody Type
- Host Species
- Partial recombinant human RAP1 protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
IP, ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 µg/test. For immunocytochemistry, a concentration of 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone does not recognize mouse RAP1.
ICC testing was conducted on Raji cells fixed with 4% paraformaldehyde and permeabilized with either Triton X-100 or methanol. Both methods were compatible with RAP1 staining.
AB_2876760 (BioLegend Cat. No. 938301)
AB_2876760 (BioLegend Cat. No. 938302)
- RAP1 is a 399 amino acid protein with an expected molecular weight of 55 kD.
- Transcription, Transcription regulation
- Biology Area
- Cell Biology, DNA Repair/Replication
- Antigen References
- Cai Y, et al. 2017. Cell Cycle. 16:1765-1773.
- Kotla S, et al. 2019. JCI Insight. 4(9):124867.
- Rai R, et al. 2015. Nat Commun. 7:10881.
- Zhang Y, et al. 2015. Cell Death Discov. 1:15007.
- Gene ID
- 54386 View all products for this Gene ID
- View information about RAP1 on UniProt.org
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