Purified anti-pig CD4a Antibody

Pricing & Availability
Clone
74-12-4 (See other available formats)
Regulatory Status
RUO
Other Names
T-cell surface antigen T4/Leu-3
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
74-12-4_PURE_CD4a_Antibody_v2_062421.png
Porcine peripheral blood lymphocytes were stained with anti-swine CD3 Alexa Fluor® 647 and purified anti-pig CD4a (clone 74-12-4) (left), or purified mouse IgG2b, κ isotype control (right), followed by anti-mouse IgG FITC.
  • 74-12-4_PURE_CD4a_Antibody_v2_062421.png
    Porcine peripheral blood lymphocytes were stained with anti-swine CD3 Alexa Fluor® 647 and purified anti-pig CD4a (clone 74-12-4) (left), or purified mouse IgG2b, κ isotype control (right), followed by anti-mouse IgG FITC.
Cat # Size Price Quantity Check Availability Save
250002 100 µg 140,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD4 is a 55 kDa, type I glycoprotein expressed by tymocytes, helper T cells (Th), plasmacytoid dendritic cells (also known as natural interferon-producing cells or NIPC). In Pigs there a CD4+/CD8+ double positive subset that increase with age and have characteristics of memory T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Pig
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Swine thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 74-12-4 recognizes the allele CD4.A in swine T cells.

Application References

(PubMed link indicates BioLegend citation)
  1. Pescovitz, M.D. et al. 1984. J. Immunol. 133:368 (FC, IP)
  2. Pescovitz, M.D. et al. 1985. J. Immunol. 134:37 (FC)
  3. Saalmuller, A., et al. 1987. Eur. J. Immunol. 17:1297 (FC)
RRID
AB_2894685 (BioLegend Cat. No. 250002)

Antigen Details

Structure
55 kDa, type I glycoprotein
Distribution

Thymocytes, helper T cells, plasmacytoid dendritic cells

Interaction
TCR/CD3 complex
Ligand/Receptor
MHC class II
Cell Type
T cells, Tfh, Th1, Th17, Th2, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Gustafsson K, et al. 1993. J Immunol. 151:1365
  2. Blanc F, et al. 2018. Immunogenetics. 70:209
  3. Zhang F, et al. 2019. Gene. 707:22
Gene ID
404704 View all products for this Gene ID
UniProt
View information about CD4a on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05.26.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account