Purified anti-Phospho-B-Raf (Thr598/Ser601) Antibody

Pricing & Availability
Clone
Poly6327 (See other available formats)
Other Names
B-Raf proto-oncogene serine/threonine-protein kinase, BRAF, p94
Isotype
Rabbit Polyclonal IgG
Ave. Rating
Submit a Review
Product Citations
publications
1-Poly6327_Pure_B-Raf_1_ICC_041519
Hela cells were fixed with 2% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/mL anti-Phospho-B-Raf (Thr598/Ser601) and followed by DyLight™ 594 goat anti-Rabbit IgG (red) for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • 1-Poly6327_Pure_B-Raf_1_ICC_041519
    Hela cells were fixed with 2% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 5 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/mL anti-Phospho-B-Raf (Thr598/Ser601) and followed by DyLight™ 594 goat anti-Rabbit IgG (red) for one hour at room temperature. Actin filaments were labeled with Alexa Fluor® 488 Phalloidin (green). Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
  • 2-Poly6327_IHC_062608
    Formalin-fixed paraffin-embedded human testis tissue was stained with Poly6327 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
Cat # Size Price Quantity Avail. Save
632702 100 µl 200€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The Raf proteins are a family of serine/threonine-specific kinases that serve as a central intermediate in transmitting extracellular signals to the mitogen-activated protein kinase cascade, which controls cell growth, differentiation and survival. Three isoforms of Raf proteins have been found in mammalian cells: Raf-1, A-Raf and B-Raf. The B-raf involved in signal transduction from the membrane to the nucleus. It has been reported that T598 and S601 are the major phosphorylation sites of B-Raf in response to oncogenic Ras, and phosphorylation of these two residues is required for full activation of B-Raf. Mutations in the B-Raf gene have been reported in a number of human cancers, including malignant melanoma, thyroid cancer, and colorectal carcinoma. The poly6327 antibody recognizes human phosphorylated B-Raf (Thr598/Ser601) and has been shown to be useful for immunoflorence staining.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Modified synthetic peptide FGLATVKSRWSGS
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by antigen-affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, store frozen at -20°C.
Application

ICC - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a dilution range of 1:100 - 1:400 is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a dilution of 1:100 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

RRID
AB_2191774 (BioLegend Cat. No. 632702)

Antigen Details

Structure
Belongs to the Raf/mil family of serine/threonine protein kinases, predicted molecular weight 84 kD
Distribution

Cytoplasm

Function
B-Raf involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It has been reported that T598 and S601 are the major phosphorylation sites of B-Raf in response to oncogenic Ras, and phosphorylation of these two residues is
Biology Area
Cell Biology
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References

1. Avruch J, et al. 1994. Trends Biochem Sci. 19:279.
2. Brose MS, et al. 2002. Cancer Res. 62:6997.
3. Zhang B-H, et al. 2000. EMBO 19:5429.

Gene ID
128260 View all products for this Gene ID
UniProt
View information about Phosphorylated B-Raf on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 02.09.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account