Purified anti-PERK Phospho (Ser713) Antibody

Pricing & Availability
Clone
Poly6494 (See other available formats)
Other Names
Eukaryotic translation initiation factor 2 alpha kinase 3, PRKR-like endoplasmic reticulum kinase, heme-regulated EIF2-alpha kinase
Isotype
Rabbit Polyclonal IgG
Ave. Rating
Submit a Review
Product Citations
publications
Poly6494_PERK_Phospho_Ser713_Antibody_WB_071715
Total cell lysate from 293T cells transfected with FLAG-PERK (lane 1) and FLAG-PERK S713A mutant (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-PERK Phospho (Ser713) antibody (clone Poly6494). Proteins were visualized using an HRP-conjugated anti-rabbit-IgG secondary and chemiluminescence detection.
  • Poly6494_PERK_Phospho_Ser713_Antibody_WB_071715
    Total cell lysate from 293T cells transfected with FLAG-PERK (lane 1) and FLAG-PERK S713A mutant (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-PERK Phospho (Ser713) antibody (clone Poly6494). Proteins were visualized using an HRP-conjugated anti-rabbit-IgG secondary and chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
649401 100 µl (5 Western blots) 100€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
649402 400 µl (20 Western blots) 280€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

PERK (protein kinase R-like endoplasmic reticulum kinase) is a type I membrane protein located in the endoplasmic reticulum (ER). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation, leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress. PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain. Phosphorylation of PERK at Ser713 can be used as a marker for its activation status. The predicted phosphorylation site and surrounding residues of Ser713 are: HIEIIAPS*PQRSRSF.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Modified synthetic peptide
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store at -20°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 20 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The optimal concentration should be determined by titration for each individual assay of interest.


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Gewandter JS, et al. 2009. Free Radic Biol Med. 47:1742. PubMed
  2. Devi, L. et al. 2012. PLoS One. 7:e32792. PubMed
  3. Zang Y, et al. 2012. Clin Cancer Res. 18:5639. PubMed
  4. Devi L, et al. 2014. Neurobiol Aging. 35:2272. PubMed
Product Citations
  1. Huang H, et al. 2011. PLoS One. 6:e22935. PubMed
  2. Devi L, et al. 2012. PLoS One. 7:e32792. PubMed
  3. Zang Y, et al. 2012. Clin Cancer Res. 18:5639. PubMed
  4. Zheng M, et al. 2013. Int Immunopharmacol. 15:517. PubMed
  5. Gewandter J, et al. 2009. Free Radic Biol Med. 47:1742. PubMed
  6. Devi L, Ohno M 2014. Neurobiol Aging. 35:2272. PubMed
  7. Tsukumo Y, et al. 2015. Biochem Biophys Res Commun. 462: 383-388. PubMed
  8. Hedrick E, et al. 2015. Endocr Relat Cancer. 22: 831-840. PubMed
  9. Tamaki T, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.03.017. PubMed
  10. Siddiquey MNA, et al. 2018. J Virol. 92:. PubMed
Publication Library
RRID
AB_10642966 (BioLegend Cat. No. 649401)
AB_10640071 (BioLegend Cat. No. 649402)

Antigen Details

Structure
1115 amino acids; 125 kD predicted. The observed MW for phosphor-PERK is approximately 150-170 kD.
Distribution

Endoplasmic reticulum membrane; Single-pass type I membrane protein

Function
Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1.
Interaction
Forms dimers with HSPA5/BIP in resting cells. Oligomerizes in ER-stressed cells.
Biology Area
Cell Biology, Protein Synthesis, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References

1. Shi Y, et al. 1998. Mol. Cell. Biol. 18:7499.
2. Shi Y, et al. 1999. J. Biol. Chem. 274:5723.
3. Cybulsky AV, et al. 2005. J. Biol. Chem. 280:24396.
4. Kohno K. 2010. J. Biochem. 147:27.

Gene ID
9451 View all products for this Gene ID
UniProt
View information about PERK Phosphorylated on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07.17.2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account