Purified anti-Pax-2 Antibody (Previously Covance catalog# PRB-276P)

Pricing & Availability
Poly19010 (See other available formats)
Regulatory Status
Other Names
Paired box protein Pax-2, paired box homeotic gene 2, Opdc, optic disc coloboma
Covance Catalog# PRB-276P
Rabbit Polyclonal IgG
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Product Citations
Pax2 staining on normal human kidney tissue, demonstrating strong nuclear staining of epithelial cells in some renal tubules.
  • 1_Purified_Pax-2_Antibody_IHC_021315
    Pax2 staining on normal human kidney tissue, demonstrating strong nuclear staining of epithelial cells in some renal tubules.
  • 2_Poly19010_PURE_Pax-2_Antibody_2_WB_112817
    Western blot analysis of cell lysates from MCF-7 (low expression negative control) and Daudi (positive control) cells using Pax-2 rabbit primary antibody (Clone Poly19010,1:1000 dilution, 1 µg/ml) and HRP Donkey anti-rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-GAPDH (Cat. No. 649203) was used as a loading control (1:1000 dilution).
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901002 25 µL 60,00€
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901001 200 µL 252,00€
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Pax genes are expressed during embryogenesis in a temporally and spatially restricted manner in the developing neural tube. Pax-2 has been identified to be involved in kidney and optic nerve development. It belongs to the Pax group 2, including Pax-5 and Pax-8. Paired box (Pax) proteins are tissue specific transcription factors that contain a paired domain and typically a partial or complete homeodomain.

Product Details
Technical Data Sheet (pdf)

Product Details

Human, Mouse, Chicken, Xenopus, Zebrafish
Antibody Type
Host Species
This Pax-2 antibody was generated against a recombinant protein containing 22kD of the Pax-2 sequence corresponding to amino acids 188-385.
Phosphate-buffered solution + 0.03% Thimerosal.
The antibody was purified by affinity chromatography.
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.

IHC-P - Quality tested
WB - Verified
IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
WB: 1-2 µg/ml (1:500-1:1000)
• Gel shifts: 1:10
IHC: 1:100

Tissue Sections: Formalin-fixed, paraffin-embedded tissues, acetone or PFA fixed frozen sections
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieva-All 1 (Cat. No. 927901).
Incubation: 20 minutes at room temperature
Staining Localization: nuclear

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), immunopurification and DNA band supershift experiments.

*Predicted MW = 44 kD
Positive Control Tissue: normal kidney or renal adenocarcinoma

This antibody recognizes Pax-2A and Pax-2B (two products of the Pax-2 gene). When linked to agarose beads, it has been used to immunoprecipitate DNA sequences recognized by Pax-2.

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Hammes A, et al. 2001. Cell. 106:319.
  2. Phelps DE, Dressler GR. 1996. J Biol Chem. 271:7978.
  3. Rothenpieler UW, Dressler GR. 1993. Development. 119:711
  4. Dressler GR, et al. 1993. Nature. 362:65
  5. Dressler GR, Douglass EC. 1992. Proc Natl Acad Sci USA. 89:1179
  6. Puschel AW, et al. 1992. Mech Dev. 38:197
  7. Riley BB, et al. 1999. Development. 126:5669. (IHC)
Product Citations
  1. Taguchi A, et al. 2017. Cell Stem Cell. 21:730. PubMed
  2. Desgrange A, et al. 2017. Development. 144:4704. PubMed
  3. Hoshi M, et al. 2018. J Am Soc Nephrol. 29:775. PubMed
  4. O'Sullivan ML, et al. 2017. Glia. 65:1697. PubMed
  5. Mattei C, et al. 2019. Front Cell Dev Biol. 7:25. PubMed
  6. Kim E, et al. 2019. Sci Rep. 9:5025. PubMed
  7. Charlton–Perkins M, et al. 2019. Glia. 67:1401. PubMed
  8. Miltner AM, et al. 2019. Int J Mol Sci. 20. PubMed
  9. Lindström NO, et al. 2018. J Am Soc Nephrol. 29:825. PubMed
  10. Haque F, et al. 2017. Dev Biol. 427:121. PubMed
  11. Tan Z, et al. 2018. Sci Rep. 8:16618. PubMed
  12. Zhang H, et al. 2019. Dev Cell. 48:780. PubMed
  13. Tulloch AJ et al. 2019. J Comp Neurol. 527(18):2948-2972 . PubMed
  14. Conceição R, et al. 2019. Invest Ophthalmol Vis Sci. 60:4727. PubMed
  15. Chow T, et al. 2020. NPJ Regen Med. 5:7. PubMed
  16. Bernstein CS, et al. 2018. Dev Biol. 440:137. PubMed
  17. Tsujimoto H, et al. 2020. Cell Reports. 31(1):107476. PubMed
  18. Huang L, et al. 2016. Neoplasia. 18:71-81. PubMed
  19. Zhao F, et al. 2016. Sci Rep. 6:23037. PubMed
  20. Prunskaite-Hyyryläinen R, et al. 2016. Hum Mol Genet. 25: 1059 - 1073. PubMed
  21. Ohnmacht J, et al. 2016. Development. 143: 1464 - 1474. PubMed
  22. Dauleh S, et al. 2016. PLoS One. 11: 0158997. PubMed
  23. Muscat A, et al. 2016. Clin Cancer Res. 22: 3560 - 3570. PubMed
  24. Ealy M, et al. 2016. Proc Natl Acad Sci U S A. 113: 8508 - 8513. PubMed
  25. Reginensi A, et al. 2016. Nat Commun. 7:12309. PubMed
  26. McNeill H, Reginensi A 2016. J Am Soc Nephrol. 10.1681/ASN.2016060611. PubMed
  27. Atsuta Y, Takahashi Y 2016. Development. 143: 3549 - 3559. PubMed
  28. C Tao, X Zhang, 2016. Cell Rep. 17:1832-1844. PubMed
  29. Estermann MA, et al. 2020. Cell Rep. 31:107491. PubMed
  30. Bosze B, et al. 2020. J Neurosci. 40:1501. PubMed
  31. Brodie-Kommit J, et al. 2021. Science Advances. 7(11):. PubMed
  32. Chang SH, et al. 2021. eLife. 10:00. PubMed
  33. Gong C, et al. 2021. Cell Reports. 34(12):108889. PubMed
  34. Sasaki K, et al. 2021. Cell Reports. 35(5):109075. PubMed
  35. Lindstrm NO, et al. 2021. Developmental Cell. 56(16):2381-2398.e6. PubMed
  36. Sun WR, et al. 2020. Hum Mol Genet. . PubMed
  37. Cleal L, et al. 2021. Dis Model Mech. :14. PubMed
  38. Omotehara T, et al. 2020. Dev Dyn. 249:754. PubMed
AB_2734656 (BioLegend Cat. No. 901002)
AB_2565001 (BioLegend Cat. No. 901001)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology, Transcription Factors
Molecular Family
Nuclear Markers
Gene ID
5076 View all products for this Gene ID
View information about Pax-2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 11.28.2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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