- TER-119 (See other available formats)
- Regulatory Status
- Other Names
- Rat IgG2b, κ
- Ave. Rating
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- Product Citations
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The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Day-14 fetal liver cells from a C57BL/6 mouse
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- The antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
CyTOF® - Verified
- Recommended Usage
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).
- Additional Product Notes
Maxpar® is a registered trademark of Standard BioTools Inc.
(PubMed link indicates BioLegend citation)
- Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
- Vannucchi AM, et al. 2000. Blood 95:2559.
- Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
- Grisendi S, et al. 2005. Nature 437:147. (FC)
- Bourdeau A, et al. 2007. Blood 109:4220.
- Chappaz S, et al. 2007. Blood 110:3862. (FC)
- Heuser M, et al. 2007. Blood 110:1639. (FC)
- Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
- Product Citations
AB_2563789 (BioLegend Cat. No. 116241)
- Associated with glycophorin A, 52 kD
Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E
- Cell Type
- Biology Area
- Antigen References
1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.
- Gene ID
- 104231 View all products for this Gene ID
- View information about TER-119 on UniProt.org
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse TER-119/Erythroid Cells
Biotin anti-mouse TER-119/Erythroid Cells
FITC anti-mouse TER-119/Erythroid Cells
PE anti-mouse TER-119/Erythroid Cells
PE/Cyanine5 anti-mouse TER-119/Erythroid Cells
Purified anti-mouse TER-119/Erythroid Cells
Alexa Fluor® 488 anti-mouse TER-119/Erythroid Cells
Alexa Fluor® 647 anti-mouse TER-119/Erythroid Cells
Alexa Fluor® 700 anti-mouse TER-119/Erythroid Cells
PE/Cyanine7 anti-mouse TER-119/Erythroid Cells
APC/Cyanine7 anti-mouse TER-119/Erythroid Cells
PerCP anti-mouse TER-119/Erythroid Cells
PerCP/Cyanine5.5 anti-mouse TER-119/Erythroid Cells
Brilliant Violet 421™ anti-mouse TER-119/Erythroid Cells
Pacific Blue™ anti-mouse TER-119/Erythroid Cells
Brilliant Violet 650™ anti-mouse TER-119/Erythroid Cells
Brilliant Violet 510™ anti-mouse TER-119/Erythroid Cells
Brilliant Violet 605™ anti-mouse TER-119/Erythroid Cells
Purified anti-mouse TER-119/Erythroid Cells (Maxpar® Ready)
PE/Dazzle™ 594 anti-mouse TER-119/Erythroid Cells
Brilliant Violet 785™ anti-mouse TER-119/Erythroid Cells
TotalSeq™-A0122 anti-mouse TER-119/Erythroid Cells
APC/Fire™ 750 anti-mouse TER-119/Erythroid Cells
TotalSeq™-B0122 anti-mouse TER-119/Erythroid Cells
TotalSeq™-C0122 anti-mouse TER-119/Erythroid Cells
Ultra-LEAF™ Purified anti-mouse TER-119/Erythroid Cells
Spark Blue™ 550 anti-mouse TER-119/Erythroid Cells
APC/Fire™ 810 anti-mouse TER-119/Erythroid Cells
Spark NIR™ 685 anti-mouse TER-119/Erythroid Cells Antibody