- AF6-88.5 (See other available formats)
- Regulatory Status
- Other Names
- MHC class I
- Mouse (BALB/c) IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kb haplotype. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).Product Details
- Mouse b haplotype
- Antibody Type
- Host Species
- C57BL mouse splenocytes
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections.
Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v).
(PubMed link indicates BioLegend citation)
- Loken MR, et al. 1982. J. Immunol. Methods 50:R85.
- Wall KA, et al. 1983. J. Immunol. 131:1056.
- Andersson M, et al. 1998. J. Immunol. 161:6475.
- Shao H, et al. 2005. J. Immunol. 175:1851.
- Hui S, et al. 2005. J. Immunol. 175:1851. PubMed
- Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed
- Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed
- Product Citations
AB_313728 (BioLegend Cat. No. 116501)
AB_313729 (BioLegend Cat. No. 116502)
- MHC class I
Nucleated cells of the H-2Kb haplotype
- Antigen presentation
- CD3/TCR, CD8
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- MHC Antigens
- Antigen References
1. Watts C. 1997. Ann. Review Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.
- Gene ID
- 14972 View all products for this Gene ID
- View information about H-2Kb on UniProt.org
|PE/Cyanine7 anti-mouse H-2Kb||AF6-88.5||FC|
|APC anti-mouse H-2Kb||AF6-88.5||FC|
|Biotin anti-mouse H-2Kb||AF6-88.5||FC|
|FITC anti-mouse H-2Kb||AF6-88.5||FC|
|PE anti-mouse H-2Kb||AF6-88.5||FC|
|Alexa Fluor® 700 anti-mouse H-2Kb||AF6-88.5||FC|
|Alexa Fluor® 488 anti-mouse H-2Kb||AF6-88.5||FC|
|Alexa Fluor® 647 anti-mouse H-2Kb||AF6-88.5||FC|
|Brilliant Violet 510™ anti-mouse H-2Kb||AF6-88.5||FC|
|Pacific Blue™ anti-mouse H-2Kb||AF6-88.5||FC|
|PerCP/Cyanine5.5 anti-mouse H-2Kb||AF6-88.5||FC|
|Brilliant Violet 421™ anti-mouse H-2Kb||AF6-88.5||FC|
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Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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PE/Cyanine7 anti-mouse H-2Kb
APC anti-mouse H-2Kb
Biotin anti-mouse H-2Kb
FITC anti-mouse H-2Kb
PE anti-mouse H-2Kb
Alexa Fluor® 700 anti-mouse H-2Kb
Alexa Fluor® 488 anti-mouse H-2Kb
Alexa Fluor® 647 anti-mouse H-2Kb
Brilliant Violet 510™ anti-mouse H-2Kb
Pacific Blue™ anti-mouse H-2Kb
PerCP/Cyanine5.5 anti-mouse H-2Kb
Brilliant Violet 421™ anti-mouse H-2Kb