- PO3 (See other available formats)
- Regulatory Status
- Other Names
- B7-2, B70, Ly-58
- Rat IgG2b, κ
- Ave. Rating
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- Product Citations
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CD86 is an 80 kD immunoglobulin superfamily member, also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells and astrocytes. CD86 along with CD80 are the ligands of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. The PO3 antibody has been shown to block autoantibody production in vivo and inhibit T cell activation in vitro.Product Details
- Antibody Type
- Host Species
- BALB/c mouse B leukemia cell line BCL1
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking 1,4,5 of autoantibody production and T cell activation, stimulation of B cell activity3, immunoprecipitation2, and immunohistochemical staining2 of acetone-fixed frozen sections.
(PubMed link indicates BioLegend citation)
- Nakajima A, et al. 1995. Eur. J. Immunol. 25:3060.
- Nuriya S, et al. 1996. Int. Immunol. 8:917.
- Kasprowicz DJ, et al. 2000. J. Immunol. 165:680.
- Saito K, et al. 1998. J. Immunol. 160:4225.
- Nakajima A, et al. 1998. J. Immunol. 161:1901.
- Ma XT, et al. 2006. Cancer Research 66:1169.
- Lawson BR, et al. 2007. J. Immunol. 178:5366.
- Bhatnagar S and Schorey JS. 2007. J. Biol. Chem. doi:10.1074/jbc.M702277200.
- Giroux M, et al. 2007. J. Immunol. 179:4492.PubMed
- Product Citations
AB_313155 (BioLegend Cat. No. 105102)
- Ig superfamily, 80 kD
B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes
- T cell costimulation, Ig class-switching, NK cell cytotoxicity
- CD28, CD152 (CTLA-4)
- Cell Type
- Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science. 262:905.
3. Freeman GJ, et al. 1993. Science. 262:907.
4. Carreno BM, et al. 2002. Annu Rev Immunol. 20:29.
- Gene ID
- 12524 View all products for this Gene ID
- View information about CD86 on UniProt.org
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