Purified anti-mouse CD86 Antibody

Pricing & Availability
Clone
PO3 (See other available formats)
Regulatory Status
RUO
Other Names
B7-2, B70, Ly-58
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
PO3_Purified_083107
LPS-stimulated (2 days) C57BL/6 mouse splenocytes stained with purified PO3, followed by anti-rat IgG FITC
  • PO3_Purified_083107
    LPS-stimulated (2 days) C57BL/6 mouse splenocytes stained with purified PO3, followed by anti-rat IgG FITC
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105102 500 µg 156,00€
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Description

CD86 is an 80 kD immunoglobulin superfamily member, also known as B7-2, B70, and Ly-58. CD86 is expressed on activated B and T cells, macrophages, dendritic cells and astrocytes. CD86 along with CD80 are the ligands of CD28 and CD152 (CTLA-4). CD86 is expressed earlier in the immune response than CD80. CD86 has also been shown to be involved in immunoglobulin class-switching and triggering of NK cell-mediated cytotoxicity. CD86 binds to CD28 to transduce co-stimulatory signals for T cell activation, proliferation, and cytokine production. CD86 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells. The PO3 antibody has been shown to block autoantibody production in vivo and inhibit T cell activation in vitro.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse B leukemia cell line BCL1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking 1,4,5 of autoantibody production and T cell activation, stimulation of B cell activity3, immunoprecipitation2, and immunohistochemical staining2 of acetone-fixed frozen sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakajima A, et al. 1995. Eur. J. Immunol. 25:3060.
  2. Nuriya S, et al. 1996. Int. Immunol. 8:917.
  3. Kasprowicz DJ, et al. 2000. J. Immunol. 165:680.
  4. Saito K, et al. 1998. J. Immunol. 160:4225.
  5. Nakajima A, et al. 1998. J. Immunol. 161:1901.
  6. Ma XT, et al. 2006. Cancer Research 66:1169.
  7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  8. Bhatnagar S and Schorey JS. 2007. J. Biol. Chem. doi:10.1074/jbc.M702277200.
  9. Giroux M, et al. 2007. J. Immunol. 179:4492.PubMed
Product Citations
  1. Paraiso HC, et al. 2018. J Neuroinflammation. 0.694444444. PubMed
  2. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  3. Giroux M, et al. 2007. J Immunol. 179:4492. PubMed
RRID
AB_313155 (BioLegend Cat. No. 105102)

Antigen Details

Structure
Ig superfamily, 80 kD
Distribution

B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes

Function
T cell costimulation, Ig class-switching, NK cell cytotoxicity
Ligand/Receptor
CD28, CD152 (CTLA-4)
Cell Type
Astrocytes, B cells, Dendritic cells, Macrophages, T cells, Tregs
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Hathcock KS, et al. 1993. Science. 262:905.
3. Freeman GJ, et al. 1993. Science. 262:907.
4. Carreno BM, et al. 2002. Annu Rev Immunol. 20:29.

Gene ID
12524 View all products for this Gene ID
UniProt
View information about CD86 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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