Purified anti-mouse CD68 Antibody

Pricing & Availability
Clone
FA-11 (See other available formats)
Regulatory Status
RUO
Other Names
Macrosialin
Isotype
Rat IgG2a
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Product Citations
publications
FA-11_A647_021810
Thioglycolate-elicited Balb/c peritoneal macrophages intracellularly stained with FA-11 Alexa Fluor® 647
  • FA-11_A647_021810
    Thioglycolate-elicited Balb/c peritoneal macrophages intracellularly stained with FA-11 Alexa Fluor® 647
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137001 50 µg 81€
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137002 500 µg 344€
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Description

Mouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Purified Con A receptor glycoproteins from the P815 cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
FC - Verified
IP, WB, IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2, immunohistochemical staining of frozen sections2 and paraformaldehyde-fixed paraffin-embedded sections3, and spatial biology (IBEX)9,10.

Application References

(PubMed link indicates BioLegend citation)
  1. Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB)
  2. Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC)
  3. Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC)
  4. Kayama H, et al. 2012. PNAS. 109:5010. PubMed
  5. Park S, et al. 2013. Biomaterials. 34:598. PubMed
  6. Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
  7. McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed
  8. Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed
  9. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Milasan A, et al. 2017. J Am Heart Assoc. 10.1161/JAHA.117.006892. PubMed
  2. Filipello F et al. 2018. Immunity. 48(5):979-991 . PubMed
  3. Miho Sato‐Hashimoto et al. 2019. eLife. 8 pii: e42025. PubMed
  4. Preda MB, et al. 2021. Cell Death Dis. 12:566. PubMed
  5. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  6. Mathew DJ, et al. 2021. Sci Rep. 11:22880. PubMed
  7. Da Mesquita S, et al. 2021. Nature. 593:255. PubMed
  8. Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
  9. Zaman R, et al. 2021. Immunity. :. PubMed
  10. Fang W, et al. 2020. Transl Res. 222:28. PubMed
  11. Spencer WJ, et al. 2019. Proc Natl Acad Sci U S A. 116:13087. PubMed
  12. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  13. Guimbal S, et al. 2021. Arterioscler Thromb Vasc Biol. 41:e193. PubMed
  14. McKinstry S, et al. 2014. J Neurosci. 34:9455. PubMed
  15. Henjum K, et al. 2020. Sci Rep. 10:10590. PubMed
  16. Sherafat A, et al. 2021. Nat Commun. 12:2265. PubMed
  17. Park S, et al. 2013. Biomaterials. 34:598. PubMed
  18. Trus I, et al. 2020. Front Immunol. 2.553472222. PubMed
  19. Shen L et al. 2019. Br J Pharmacol. 176(14):2559-2572 . PubMed
  20. Tian K, et al. 2021. J Immunol Res. 2021:6297332. PubMed
  21. Gomez-Pastor R, et al. 2017. Nat Commun. 8:14405. PubMed
  22. Brocker CN, et al. 2017. Am J Physiol Gastrointest Liver Physiol. 312:G283. PubMed
  23. Di Gioia M, et al. 2020. Nat Immunol. 21:42. PubMed
  24. Burrus CJ, et al. 2020. Cell Rep. 30:642. PubMed
  25. Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed
  26. Mariot V, et al. 2021. J Cachexia Sarcopenia Muscle. Online ahead of print. PubMed
  27. O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
  28. Batool M, et al. 2020. Basic Res Cardiol. 115:68. PubMed
  29. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  30. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  31. Silburt J, et al. 2022. J Neuroinflammation. 19:24. PubMed
  32. Kim H, et al. 2020. ACS Nano. 14:6519. PubMed
  33. Mori H, et al. 2015. Toxicol Pathol. 43: 883-889. PubMed
  34. Goldblatt DL, et al. 2020. British Journal of Pharmacology. 177(10):2256-2273.. PubMed
  35. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  36. Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed
  37. Carvalho K, et al. 2022. J Neuroinflammation. 19:178. PubMed
  38. Stafeev I, et al. 2022. Front Nutr. 8:809732. PubMed
  39. Shi W, et al. 2021. Biosci Rep. 41:. PubMed
  40. Godoy-Calderón MJ, et al. 2018. Oncotarget. 8:11370. PubMed
  41. Wu Y, et al. 2019. Nat Commun. 10:58. PubMed
  42. Cornelison RC, et al. 2022. NPJ Precis Oncol. 6:54. PubMed
  43. Collier AF, et al. 2018. J Pharmacol Exp Ther. 364:409. PubMed
  44. Choi SH, et al. 2020. Arterioscler Thromb Vasc Biol. :ATVBAHA120315485. PubMed
  45. Wang L, et al. 2020. Sci Rep. 10:16326. PubMed
  46. Cushing EM, et al. 2018. J Lipid Res. 59:1230. PubMed
  47. Ikezoe T, et al. 2021. Biomolecules. 11:. PubMed
  48. Koch PS, et al. 2021. Front Physiol. 12:722394. PubMed
  49. Milasan A, et al. 2019. Atherosclerosis. 283:106. PubMed
  50. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  51. Tsai MS, et al. 2021. Int J Mol Sci. 22:. PubMed
  52. Phua T, et al. 2017. Sci Rep. 10.1038/srep44351. PubMed
  53. Cheng X, et al. 2021. Redox Biol. 40:101863. PubMed
  54. Ni K, et al. 2018. Respir Res. 0.865972222. PubMed
  55. Dong Y, et al. 2021. STAR Protoc. 2:100853. PubMed
  56. Yue F, et al. 2021. Mol Ther. 29:132. PubMed
  57. Han X, et al. 2020. Mol Carcinog. 59:1088. PubMed
  58. Healy A, et al. 2020. Arterioscler Thromb Vasc Biol. 40:714. PubMed
  59. Kayama H, et al. 2012. Proc Natl Acad Sci U S A. 109:5010. PubMed
RRID
AB_2044003 (BioLegend Cat. No. 137001)
AB_2044004 (BioLegend Cat. No. 137002)

Antigen Details

Structure
A member of the lysosomal-associated membrane protein (lamp) family.
Distribution

Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.

Function
Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
Cell Type
Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules, Innate Immune Signaling
Antigen References

1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833.
2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.

Gene ID
12514 View all products for this Gene ID
UniProt
View information about CD68 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04.26.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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