Purified anti-mouse CD64 (FcγRI) Antibody

Pricing & Availability
Clone
S18017D (See other available formats)
Regulatory Status
RUO
Other Names
FcRI
Isotype
Rat IgG2a, κ
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Product Citations
publications
S18017D_PURE_CD64_FcgammaRI_Antibody_030821
C57BL/6 mouse bone marrow cells were stained with anti-mouse CD11b APC and purified anti-mouse CD64 (FcγRI) (clone S18017D) (left) or purified anti-rat IgG2a, κ isotype control (right), followed by goat anti-rat IgG PE. Data shown was gated on myeloid cells.
  • S18017D_PURE_CD64_FcgammaRI_Antibody_030821
    C57BL/6 mouse bone marrow cells were stained with anti-mouse CD11b APC and purified anti-mouse CD64 (FcγRI) (clone S18017D) (left) or purified anti-rat IgG2a, κ isotype control (right), followed by goat anti-rat IgG PE. Data shown was gated on myeloid cells.
  • S18017D_PURE_CD64_FcgammaRI_Antibody_2_102821
  • S18017D_PURE_CD64_FcgammaRI_Antibody_3_102821
    C57BL/6 mouse frozen liver section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then, the section was stained with 10 µg/mL of Purified anti-mouse CD64 (FcγRI) Antibody (clone S18017D) overnight at 4°C. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 anti-rat IgG (clone Poly4054, red) for two hours at room temperature. The image was captured with a 10X objective.
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161002 100 µg 80,00€
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Description

CD64 is a 72 kD single chain type I glycoprotein also known as FcγRI and FcRI. CD64 is a member of the immunoglobulin superfamily and is expressed on monocytes/macrophages, dendritic cells, and mast cells. The expression can be upregulated by IFN-γ stimulation. CD64 binds IgG immune complex. It plays a role in antigen capture, phagocytosis of IgG/antigen complexes, and antibody-dependent cellular cytotoxicity (ADCC).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse FcγRI/CD64 (Cat. No. 773806)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2890725 (BioLegend Cat. No. 161002)

Antigen Details

Structure
Ig superfamily, type I glycoprotein, 72 kD
Distribution

Monocytes, macrophages, mast cells, dendritic cells

Function
Phagocytosis, ADCC
Ligand/Receptor
IgG
Antigen References

1. Schyns J, et al. 2019. Nat. Commun. 3;10(1):3964
2. Langlet C, et al. 2012. J. Immunol. 188 (4) 1751-1760.

Gene ID
14129 View all products for this Gene ID
UniProt
View information about CD64 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10.28.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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