- 9C10 (MFR4.B) (See other available formats)
- Regulatory Status
- Other Names
- α4 integrin, VLA-4 α chain, integrin α4, ITGA4
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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CD49d is a 150 kD glycoprotein, also known as α4 integrin or VLA-4 α chain. It is a member of the integrin family, expressed on T and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, and dendritic cells. CD49d is a heterodimer expressed with either of two β chains, β1 (CD29) or β7, to form the VLA-4 (integrin α4β1) or LPAM-1 (integrin α4β7) complexes. CD49d plays a critical role in both adhesion and T cell costimulation. The primary ligands for CD49d are VCAM-1, MAdCAM-1, and fibronectin. The 9C10(MFR4.B) antibody, in combination with the R1-2 monoclonal antibody, completely blocks VCAM-1 binding to VLA-4.Product Details
- Antibody Type
- Host Species
- (C57BL/6 x A/J)F1 mouse fetal liver mas
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
Block, IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: in vitro blocking of cell-cell adhesion and T cell costimulation1,2, and immunohistochemistry3 of acetone-fixed frozen sections. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103709 & 103710).
(PubMed link indicates BioLegend citation)
- Kinashi T, et al. 1994. Blood Cells 20:25. (Block)
- Halvorson MJ, et al. 1995. J. Immunol. 155:4567. (Block)
- Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
- Jia W, et al. 2005. Blood 106:3854. (FC)
- Product Citations
AB_313042 (BioLegend Cat. No. 103701)
- Integrin family, associates with integrin β1 or β7, 150 kD
T cells and B cells, monocytes, eosinophils, basophils, mast cells, thymocytes, NK cells, dendritic cells
- T cell costimulation, cell-cell and cell-matrix adhesion
- VCAM-1, MAdCAM-1, fibronectin
- Cell Type
- B cells, Basophils, Dendritic cells, Eosinophils, Mast cells, Monocytes, NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lobb RR, et al. 1994. J. Clin. Invest. 94:1722.
3. Berlin C, et al. 1993. Cell 74:185.
4. Maguire JE, et al. 1995. J. Exp. Med. 182:2079.
- Gene ID
- 16401 View all products for this Gene ID
- View information about CD49d on UniProt.org
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