Purified anti-mouse CD44 Antibody

Pricing & Availability
Clone
W19210B (See other available formats)
Regulatory Status
RUO
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
A_W19210B_PURE_CD44_Antibody_3_060122
Mouse splenocytes were stained with anti-mouse CD62L APC and purified anti-mouse CD44 (clone W19210B) (left) or rat IgG2a, κ isotype control (right) followed by PE anti-rat IgG.
  • A_W19210B_PURE_CD44_Antibody_3_060122
    Mouse splenocytes were stained with anti-mouse CD62L APC and purified anti-mouse CD44 (clone W19210B) (left) or rat IgG2a, κ isotype control (right) followed by PE anti-rat IgG.
  • B_W19210B_PURE_CD44_Antibody_1_060122
    ICC staining of purified anti-mouse CD44 (clone W19210B) on NIH-3T3 cells. The cells were fixed with 4% PFA, and permeabilized with 100% ice-cold methanol. Cells were then blocked with 5% fetal bovine serum in PBS for 30 minutes, and then incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-rat IgG (red) (Cat. No. 405422) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slides were mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale bar: 50 µm
  • C_W19210B_PURE_CD44_Antibody_2_060122
    IHC staining using purified anti-mouse CD44 (clone W19210B) on formalin-fixed frozen mouse spleen tissue. The tissue was incubated with 5 μg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-rat IgG (yellow) (Cat. No. 405416) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 10x objective.
Cat # Size Price Quantity Check Availability Save
949601 25 µg 88€
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949602 100 µg 220€
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Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant mouse CD44 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC, IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. For immunohistochemical staining on frozen tissue sections, a concentration range of 1.125 - 5.0 µg/mL is suggested It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Not recommended for western blotting. Not reactive for human CD44 (tested in WB and IHC-P). 

RRID
AB_2922650 (BioLegend Cat. No. 949601)
AB_2922650 (BioLegend Cat. No. 949602)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Haynes BF, et al. 1991. Cancer Cells. 3:347-50.
  3. Goldstein LA, et al. 1989. Cell. 56:1063-72.
  4. Mikecz K, et al. 1995. Nat Med. 1:558-63.
  5. Hegde V, et al. 2008. J Leukoc Biol. 84:134-42.
  6. Liu T, et al. 2009. Biol Direct. 4:40.
Gene ID
960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05.13.2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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