- B3B4 (See other available formats)
- Regulatory Status
- Other Names
- FcεRII, IgE Fc Receptor, BLAST-2, Ly-42, B6
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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CD23 is a 45 kD protein also known as low affinity IgE Fc receptor, FcεRII, BLAST-2, Ly-42, or B6. It is a member of the Ig family, expressed on conventional B (but not B-1) cells and follicular dendritic cells. CD23 responds to high levels of IgE by downregulating IgE secretion.Product Details
- Antibody Type
- Host Species
- Complex of IgE with Fcε receptor isolated from the mouse B hybridoma cell line O1.2B2
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- The antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
CyTOF® - Verified
- Recommended Usage
This product is suitable for use with the Maxpar® Metal Labeling Kits. The product is formulated to simplify the antibody preparation when performing the labeling protocol. As a result, it is possible to proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
The B3B4 antibody is useful for blocking IgE activity in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunofluorescence microscopy, in vitro and in vivo blocking of ligand binding2-4, and immunohistochemical staining of acetone-fixed frozen sections5.
- Additional Product Notes
Maxpar® is a registered trademark of Fluidigm Inc.
(PubMed link indicates BioLegend citation)
- Waldschmidt TJ, et al. 1988. J. Immunol. 140:2148. (IP)
- Rao M, et al. 1987. J. Immunol. 138:1845. (Block)
- Oshiba A, et al. 1997. J. Immunol. 159:4056. (Block)
- Dasic G, et al. 1999. Eur. J. Immunol. 29:2957. (Block)
- Maeda K, et al. 1992. J. Immunol. 148:2340. (IHC)
- Craig VJ, et al. 2011. Cancer Res. 71:3616. PubMed
AB_2563731 (BioLegend Cat. No. 101625)
- C-lectin family, type II transmembrane glycoprotein, 45 kD
B cells, follicular dendritic cells
- Regulates B cell activation
- IgE, CD19/CD21/CD81
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Delespesse G, et al. 1992. Immunol. Rev. 125:77.
3. Flores-Romo L, et al. 1993. Science 261:1038.
- Gene ID
- 14128 View all products for this Gene ID
- View information about CD23 on UniProt.org
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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