Purified anti-Merlin (NF2) Antibody

Pricing & Availability
Clone
W19231A (See other available formats)
Other Names
Neurofibromin 2, NF2, Merlin, Schwannomin, SCH, Neurofibromin 2 (Bilateral Acoustic Neuroma), Moesin-Ezrin-Radixin-Like Protein, Moesin-Ezrin-Radixin Like, Schwannomerlin, BANF, ACN
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W19231A_PURE_Merlin_Antibody_1_040721.png
Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL purified anti-Merlin antibody (clone W19231A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker.
  • W19231A_PURE_Merlin_Antibody_1_040721.png
    Whole cell extracts (15 µg total protein) from the indicated cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL purified anti-Merlin antibody (clone W19231A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker.
  • W19231A_PURE_Merlin_Antibody_2_040721.png
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg of purified rat IgG2b, κ isotype ctrl antibody (Cat. No. 400602) or purified anti-Merlin antibody (clone W19231A). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a control antibody raised against a separate epitope of Merlin. Lane M: Molecular weight marker.
Cat # Size Price Quantity Avail. Save
945201 25 µg 80€
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945202 100 µg 200€
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Description

Moesin-ezrin-radixin-like protein (Merlin) is a tumor suppressor protein encoded by the Neurofibromin 2 (NF2) gene. It functions as a scaffold linking the plasma membrane and the cytoskeleton, and plays a crucial role in regulating cell polarization and morphology. Merlin functions as a major upstream activator of the Hippo signaling pathway, where it promotes Lats1/2 degradation, leading to increased nuclear localization of the YAP/TAZ transcription factors. Mutations and deletions within the NF2 gene cause neurofibromatosis type 2, a tumorigenic disease of the brain.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Synthetic peptide corresponding to human Merlin.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using 4% PFA-fixed HeLa cells permeabilized with either methanol or Triton X-100, and methanol-fixed and permeabilized HeLa cells. None of the methods were compatible with Merlin staining.

This clone is not predicted to cross-react with other Merlin homologs due to poor sequence conservation between the immunizing peptide and the corresponding region in each homolog.

Antigen Details

Structure
Merlin is a 595 amino acid protein with a predicted molecular weight of 70 kD.
Distribution

Ubiquitously expressed/Cytosol

Function
Tumor suppression, apoptosis
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure
Antigen References
  1. Li W, et al. 2010. Cell. 140:477-490
  2. Petrilli A, et al. 2016. Oncogene. 35:537-548.
  3. White S, et al. 2019. Dev Cell. 49:425-443.E9.
Gene ID
4771 View all products for this Gene ID
UniProt
View information about Merlin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04.07.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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