Disks large homolog 4, discs large homolog 4, Tax interaction protein 15, synapse-associated protein 90, postsynaptic density protein 95, post-synaptic density protein 95
IHC staining of purified anti-MAGUK (pan reactive) antibody (clone K28/86) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of purified anti-MAGUK (pan reactive) antibody (clone K28/86) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of purified anti-MAGUK (pan reactive) antibody (clone K28/86) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
IHC staining of purified anti-MAGUK (pan reactive) antibody (clone K28/86) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
IHC staining of purified anti-MAGUK (pan reactive) antibody (clone K28/86) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin HRP kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Western blot of purified anti-MAGUK (pan reactive) antibody (clone K28/86). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
Western blot of purified anti-MAGUK antibody (K28/86). Lane 1: Molecular weight marker; Lane 2: 20 μg of Drosophila head lysate; Lane 3: 20 μg of Drosophila S2 (embryonic) cell lysate. The blot was incubated with 0.5 μg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 436302). Enhanced chemiluminescence was used as the detection system.
Membrane-associated guanylate kinases (MAGUK) are a superfamily of scaffolding proteins defined by the presence of a guanylate kinase (GUK) domain. MAGUKs, such as PSD-95, SAP97, PSD-83, CASK, and MAGIs are involved in the synaptic development and function where they are important for the spatial organization of both pre- and postsynaptic proteins, including glutamate and other receptors.
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 77-299 of human PSD-95.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 1.0 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 0.5 - 10.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes
This antibody is less reactive in human tissue in IHC-P.
Reactivity to Drosophila was only verified with the purified format.
Additional Product Notes
For Western blotting, the suggested use of this reagent is 1 - 10 µg/ml in human, mouse, and rat and 0.5 - 10 µg/ml in Drosophila.
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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