- K8 (See other available formats)
- Regulatory Status
- Other Names
- Sialic acid-binding Ig-like lectin-9, CD329
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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Siglecs are cell surface receptors belonging to the immunoglobulin superfamily that recognize sugar antigens. The extracellular domain of siglec-9 contains an IgV region, which binds sialic acid, followed by two IgC regions. Siglec 9 and siglec 6-8,10-12 are CD33 (siglec 3) like siglecs, which have two ITIMs in the cytoplasmic tails, suggesting their functional involvement in signal transduction. It is highly expressed on neutrophils and monocytes, and at lower levels on the subpopulations of T and B lymphocytes and NK cells. Siglec-9 plays a role in negative regulation of T cell activation, and it also affects neutrophil apoptosis.Product Details
- Antibody Type
- Host Species
- Recombinant Siglec-9 fused to Fc region of human IgG
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
ELISA, ICC, IP, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence staining2, Western blotting2, immunoprecipitation2, and ELISA3.
(PubMed link indicates BioLegend citation)
- Zhang J, et al. 2000. J. Biol. Chem. 275:22121.
- Avril T, et al. 2004. J. Immunol. 173:6841. (IF, IP, WB)
- Biedermann B, et al. 2007. Leukemia Res. 31:221. (ELISA)
- Product Citations
AB_10896430 (BioLegend Cat. No. 351502)
- Immunoglobulin superfamily
Highly expressed on neutrophils and monocytes, and at lower levels on the subpopulations of T cells and B lymphocytes and NK cells
- Sialic acid
- Cell Type
- Monocytes, Neutrophils
- Biology Area
- Immunology, Innate Immunity, Costimulatory Molecules
- Molecular Family
- CD Molecules, Siglec Molecules
- Antigen References
1. Ikehara Y, et. al. 2004. J. Biol. Chem. 279:43117.
2. von Gunten S, et al. 2005. Blood 106:1423.
- Gene ID
- 27180 View all products for this Gene ID
- View information about Siglec-9 on UniProt.org
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