Purified anti-human/mouse ZAP-70 Antibody

Pricing & Availability
Clone
1E7.2 (See other available formats)
Other Names
Zeta chain associated protein kinase, SYK related tyrosine kinase
Isotype
Mouse IgG1
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Product Citations
publications
Zap-70_080304
Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-ZAP-70 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
  • Zap-70_080304
    Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with monoclonal anti-ZAP-70 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
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313402 100 µg 124€
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Description

ZAP-70 is a 70 kD protein tyrosine kinase associated with the zeta chain of the T cell receptor. It is expressed in T cells and NK cells and has been shown to be involved in T cell signaling. Defects in ZAP-70 have been linked to selective T cell defects. The ZAP-70 kinase undergoes multiple phosphorylation events after T cell receptor engagement and interacts with a number of proteins involved in signal transduction. Recently, ZAP-70 has been identified as an important prognostic marker in B-cell chronic lymphocytic leukemia (B-CLL). The 1E7.2 monoclonal antibody recognizes human and mouse ZAP-70 and has been shown to be useful for flow cytometry, Western blotting, and immunoprecipitation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
GST-fusion protein corresponding to residues 282-307 of human ZAP-70
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration 0.2 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC, WB - Quality tested
IP - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis or Western blotting. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. For Western blotting, the suggested working dilution(s)is 0.5-1 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2, Western blotting2.

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.

Application References

(PubMed link indicates BioLegend citation)
  1. Qian D, et al. 1997. J. Exp. Med. 185:1253. (ICFC)
  2. Crespo M, et al. 2003. New Engl. J. Med. 348:1764. (WB IP)
  3. Jamroziak K, et al. 2009. Cancer Epidemiol Biomarkers Prev. PubMed
  4. Tai TS, et al. 2013. J. Immunol. 190:428. PubMed.
Product Citations
  1. Jamroziak K, et al. 2009. Cancer Epidemiol Biomarkers Prev. 1.40625. PubMed
  2. Tai T, et al. 2013. J Immunol. 190:428. PubMed
RRID
AB_314995 (BioLegend Cat. No. 313402)

Antigen Details

Structure
Protein tyrosine kinase, contains two SH2 domains, 70 kD
Distribution

T cells, NK cells, B-cell chronic lymphocytic leukemia (B-CLL)

Function
Signal transduction, associates with the T-cell antigen receptor zeta chain tyrosine-based activation motif when phosphorylated. Defects in ZAP-70 causes selective T cell defect
Ligand/Receptor
Binds to CD3 zeta chain, also associates with a variety of proteins including SLA, Fyn, RasGap, Lck, Vav1, and Shc
Modification
Multiple phosphorylation sites (Tyr319 phosphorylation essential for full activity)
Cell Type
Leukemia, NK cells, T cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase, TCRs
Antigen References

1. Chan AC, et al. 1992. Cell 71:649.
2. Chan AC, et al. 1994. Science 264:1559.
3. Arpaia E, et al. 1994. Cell 76:947.

Gene ID
22637 View all products for this Gene ID 7353 View all products for this Gene ID
UniProt
View information about ZAP-70 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 08.27.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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