Purified anti-human Mast Cell Tryptase Antibody

Pricing & Availability
Clone
AA1 (See other available formats)
Other Names
Tryptase-1, β-tryptase, tryptase alpha/beta-1, TPSAB1, TPS1, TPS2, TPSB1
Isotype
Mouse IgG1, κ
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Product Citations
publications
AA1_Pure_MastCellTryptase_Antibody_022719
Human tonsil tissue was formalin fixed and paraffin embedded prior to sectioning. After deparaffinization and antigen retrieval with sodium citrate pH 6, the sample was stained overnight with anti-human Mast Cell Tryptase (clone AA1) at 10 µg/ml followed by detection using BioLegend's Ultra Streptavidin (USA) HRP detection kit.
  • AA1_Pure_MastCellTryptase_Antibody_022719
    Human tonsil tissue was formalin fixed and paraffin embedded prior to sectioning. After deparaffinization and antigen retrieval with sodium citrate pH 6, the sample was stained overnight with anti-human Mast Cell Tryptase (clone AA1) at 10 µg/ml followed by detection using BioLegend's Ultra Streptavidin (USA) HRP detection kit.
Cat # Size Price Quantity Avail. Save
369402 100 µg 156€
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Description

Mast Cell Tryptase (β-tryptase) is a neutral serine protease member of the peptidase family S1, composed of four subunits, each with a molecular weight of 32.5 kD. Mast cell tryptase has a role in inflammation and tissue remodeling. It also is a mediator in the pathogenesis of asthma and other allergic and inflammatory disorders by and its contribution to extracellular matrix-degrading processes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Monkey, Sheep (Ovine), Pig (Swine, Porcine), Cow (Cattle, Bovine), Dog (Canine), Cat (Feline), Horse (Equine)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human mast cell tryptase purified from lung tissue.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICFC, ELISA, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemistry. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Tissue Sections: Formalin-fixed, paraffin-embedded tissue sections
Primary Antibody Incubation: 60 minutes at room temperature
Antigen Retrieval: Sodium citrate pH6 (Cat# 928601)

Application Notes

Additional reported applications (for the relevant formats of this clone) include: Mast cell tryptase detection in indirect ELISA1 and Western Blot1, immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections2,3, and flow cytometric analysis of cells from bronchoalveolar lavage4.

We recommend using our Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species) (Cat# 929601).

Application References

(PubMed link indicates BioLegend citation)
  1. Walls AF, et al. 1990. Clin. Exp. Allergy 20:581. (ELISA, WB)
  2. Walls AF, et al. 1990. J. Pathol. 162:119. (IHC)
  3. Berger P, et al. 1998. Am. J. Respir. Crit. Care Med. 157:610. (IHC)
  4. Redington AE, et al. 2000. Ann. Allergy Asthma Immunol. 85:501. (IHC, ICFC)
RRID
AB_2566541 (BioLegend Cat. No. 369402)

Antigen Details

Structure
Neutral serine protease, member of the peptidase family S1, and a tetramer with a molecular weight of approximately 132 kD.
Distribution

Mast cells.

Function
Role in inflammation and tissue remodeling and may contribute to extracellular matrix-degrading processes.
Cell Type
Mast cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Abdelmotelb AM, et al. 2014. Clin. Exp. Allergy 44:822.
2. Douaiher J, et al. 2014. Adv. Immunol. 122:211.
3. Moreno M, et al. 2014. PLoS One. 9:e97014.
4. Yavuz ST, et al. 2013. Allergy 68:386.
5. Mangia A, et al. 2011. Histopathology 58:1096.

Gene ID
7177 View all products for this Gene ID
UniProt
View information about Mast Cell Tryptase on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12.29.2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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