Purified anti-human Mast Cell Tryptase Antibody

Product Details

Verified Reactivity

Human

Reported Reactivity

Monkey, Sheep, Pig, Cow, Dog, Cat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human mast cell tryptase purified from lung tissue.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

IHC-P - Quality tested
ICFC, ELISA, WB - Reported in the literature, not verified in house
SB - Community verified

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemistry. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Tissue Sections: Formalin-fixed, paraffin-embedded tissue sections
Primary Antibody Incubation: 60 minutes at room temperature
Antigen Retrieval: Sodium citrate pH6 (Cat# 928601)

Application Notes

Additional reported applications (for the relevant formats of this clone) include: Mast cell tryptase detection in indirect ELISA¹ and Western Blot¹, immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections^2,3, and flow cytometric analysis of cells from bronchoalveolar lavage⁴.

We recommend using our Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species) (Cat# 929601).

Additional Product Notes

For the use of this antibody in spatial biology applications, we have partnered with Lunaphore Technologies for demonstration of our antibodies on the COMET™. The COMET™ platform is an automated, end-to-end spatial biology solution developed for rapid and flexible multiplex tissue profiling. More information on the COMET™ and a complete list of our antibodies that have been demonstrated on the COMET™ can be found here.

Application References

(PubMed link indicates BioLegend citation)

1. Walls AF, et al. 1990. Clin. Exp. Allergy 20:581. (ELISA, WB)
2. Walls AF, et al. 1990. J. Pathol. 162:119. (IHC)
3. Berger P, et al. 1998. Am. J. Respir. Crit. Care Med. 157:610. (IHC)
4. Redington AE, et al. 2000. Ann. Allergy Asthma Immunol. 85:501. (IHC, ICFC)

Product Citations

1. Pattabiraman G, et al. 2021. Am J Physiol Renal Physiol. . PubMed

RRID

AB_2566541 (BioLegend Cat. No. 369402)

Antigen Details

Structure

Neutral serine protease, member of the peptidase family S1, and a tetramer with a molecular weight of approximately 132 kD.

Distribution

Mast cells.

Function

Role in inflammation and tissue remodeling and may contribute to extracellular matrix-degrading processes.

Cell Type

Mast cells

Biology Area

Immunology, Innate Immunity

Antigen References

1. Abdelmotelb AM, et al. 2014. Clin. Exp. Allergy 44:822.
2. Douaiher J, et al. 2014. Adv. Immunol. 122:211.
3. Moreno M, et al. 2014. PLoS One. 9:e97014.
4. Yavuz ST, et al. 2013. Allergy 68:386.
5. Mangia A, et al. 2011. Histopathology 58:1096.

Gene ID

7177 View all products for this Gene ID

UniProt

View information about Mast Cell Tryptase on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Immunohistochemistry Protocol for Paraffin-Embedded Sections

Related Pages & Pathways

Related FAQs

If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?

It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.

Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?

Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.

Are other fluorophores compatible with IBEX?

Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.

The same antibody works in one tissue type but not another. What is happening?

Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.

How can I be sure the staining I’m seeing in my tissue is real?

In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Other Formats

Certificate of Analysis