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Purified anti-human DcR3 Antibody

Pricing & Availability
Clone
B08-35 (See other available formats)
Regulatory Status
RUO
Other Names
Decoy receptor 3, TR6, M68
Isotype
Mouse IgG1, κ
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Product Citations
publications
B08-35_DcR3_Purified_Antibody_WB_012114.jpg
Western blot analysis of HCT-116 cells (lane 1) and Hela cells (lane 2) using DcR3 antibody (clone B08-35).
  • B08-35_DcR3_Purified_Antibody_WB_012114.jpg
    Western blot analysis of HCT-116 cells (lane 1) and Hela cells (lane 2) using DcR3 antibody (clone B08-35).
  • DcR3-IHC1_041306
  • DcR3-IHC2_041306
    Formalin-fixed, paraffin-embedded human tissues were stained with 5 µg/ml B08-35, followed by incubation with biotinylated goat anti-mouse secondary antibody, followed by HRP-streptavidin. Staining was visualized with DAB peroxidase substrate and counterstained with hematoxylin. Immunohistochemical staining of DcR3 expression is shown for human breast cancer (TOP) and lung adenocarcinoma (BOTTOM).
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333202 100 µg 165€
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Description

The DcR3 protein is a soluble member of the TNF receptor superfamily containing four TNF receptor domains with an approximate molecular weight of 35 kD. DcR3, also known as Decoy receptor 3, TR6, M68, can be secreted by monocytes and myeloid-derived dendritic cells following exposure to bacterial antigens. DcR3 is overexpressed in tumors including lung and colon cancers, gliomas, and pancreatic cancers and can be detected in the serum of cancer patients. DcR3 can also be found in the cytoplasm and endoplasmic reticulum in some cell types. This protein competes for ligand binding with cell surface receptor and can inhibit their signaling. DcR3 has been shown to bind the ligands FasL, LIGHT, and TNF like molecule 1 (TL1A) to inhibit binding to the cell surface receptors Fas, HVEM and LT-ßR, and death receptor 3 (DR3), respectively.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human DcR3 Fc protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP, IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5-20 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation2, and immunohistochemistry2.

Application References

(PubMed link indicates BioLegend citation)
  1. Hsu TL, et al. 2005. J. Immunol. 175:5135. (WB)
  2. Personal communication (IP IHC ELISA Detection)
  3. Tai SK, et al. 2012. J. Immunol. 188:2464. PubMed.
Product Citations
  1. Yang CY, et al. 2019. Head Neck. 41:1260. PubMed
  2. Tai S, et al. 2012. J Immunol. 188:2464. PubMed
  3. Weng S, et al. 2015. Sci Rep. 5: 12769. PubMed
RRID
AB_2207467 (BioLegend Cat. No. 333202)

Antigen Details

Structure
Soluble member of the TNF superfamily (6B), contains four TNF-receptor like domains, approximate molecular weight 35 kD.
Distribution

Secreted protein, also found in cytoplasm and endoplasmic reticulum. Expressed by monocytes and myeloid-derived dendritic cells following exposure to bacterial antigens. Overexpressed in tumors including lung and colon cancers, gliomas and pancreatic canc

Function
Competes with ligand binding to surface receptors including FasL binding to Fas, LIGHT binding to HVEM and LT-ßR, and TNF like molecule 1 (TL1A) with death receptor 3 (DR3). Competition inhibits cell-surface receptor signaling.
Interaction
Binds FasL, LIGHT, and TL1A
Cell Type
Monocytes, Dendritic cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
Cytokine/Chemokine Receptors
Antigen References

1. Pitti RM, et al. 1998. Nature 396:699.
2. Bai C, et al. 2000. Proc. Natl. Acad. Sci. USA 97:1230.
3. Yu KY, et al. 1999. J. Biol. Chem. 274:13733.
4. Migone TS, et al. 2002. Immunity 16:479.

Gene ID
8771 View all products for this Gene ID
UniProt
View information about DcR3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01.21.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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