Purified anti-human CD66d/e Antibody

Pricing & Availability
308/3-3 (See other available formats)
Regulatory Status
Other Names
Mouse IgG1, κ
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Product Citations
Human colorectal adenocarcinoma cell line HT-29 stained with D66d/e (clone 308/3-3) purified (filled histogram) or mouse IgG1, κ isotype control (clone MOPC-21) purified (open histogram).
  • 3083-3_pure_CD66de_Antibody_021918
    Human colorectal adenocarcinoma cell line HT-29 stained with D66d/e (clone 308/3-3) purified (filled histogram) or mouse IgG1, κ isotype control (clone MOPC-21) purified (open histogram).
  • 3083-3_PURE_CD66d-e_Antibody_2_IHC-P_031318
    Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate-Buffered 1X pH6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of purified anti-human CD66b (clone 308/3-3) antibody overnight at 4°C. On the next day, tissue was incubate with Alexa Fluor® 594 Goat anti-mouse IgG (clone poly4053) antibody (red). Nuclei were counter-stained with DAPI (green). The image was scanned with a 10X objective and stitched with MetaMorph® software.
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392802 100 µg 113€
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The 308/3-3 mAb reacts with an antigen epitope shared by CD66d and e. CD66d/e are members of the CEA (carcinoembryonic antigen) family of the Ig superfamily. CD66 plays a role in hemophilic and heterophilic adhesion. CD66d is a major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including NeisseriaMoraxella and Haemophilus species, thus playing an important role in the clearance of pathogens by the innate immune system. Responsible for RAC1 stimulation in the course of pathogen phagocytosis. CD66e molecule is also called CEA or CEACAM5, and is primarily found on epithelial cells. CD66e binds to CD66a, c and e.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Recombinant soluble human CEACAM3-Fc produced in HEK293 cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

AB_2728421 (BioLegend Cat. No. 392802)

Antigen Details

Ig superfamily, CEA antigen group, GPI-linked glycoprotein


Cell adhesion, neutrophil activation
CEACAM1, 5,6, SRC and ZPLD1.
CD66e: CEACAM1, 5, 6.
Cell Type
Biology Area
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Chen T, et al. 2001. J Biol Chem. 276:17413
  2. Wang Z, et al. 2016. Mol Clin Oncol. 5:731
  3. Swords DS, et al. 2016. Onco Targets Ther. 9:7459
Gene ID
1084 View all products for this Gene ID
View information about CD66d CD66e on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 02.20.2018

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD66d/e

  • Alexa Fluor® 594 anti-human CD66d/e

  • Alexa Fluor® 647 anti-human CD66d/e


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