Purified anti-human CD21 Antibody

Pricing & Availability
Bu32 (See other available formats)
Regulatory Status
V CD21.4, VI CD21.5
Other Names
Complement C3d receptor (C3dR), complement receptor 2 (CR2), Epstein-Barr virus receptor
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphocytes were stained with purified CD21 (clone Bu32) (filled histogram) or mouse IgG1, κ isotype control (dashed histogram), followed by anti-mouse IgG FITC.
  • 1_Bu32_Pure_070212
    Human peripheral blood lymphocytes were stained with purified CD21 (clone Bu32) (filled histogram) or mouse IgG1, κ isotype control (dashed histogram), followed by anti-mouse IgG FITC.
  • 2_Bu32_PURE_CD21_Antibody_IHC-P_062321.png
    IHC staining of purified anti-human CD21 antibody (clone Bu32) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 0.1 M Tris-buffered saline with Tween 20 (Cat. No. 925501), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (yellow) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 20x objective. Scale bar: 100 µm
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354902 100 µg 90€
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CD21 is a 145 kD transmembrane protein also known as complement C3d receptor (C3dR), complement receptor 2 (CR2), and Epstein-Barr virus receptor. CD21 is expressed on B cells, follicular dendritic cells, subsets of normal thymocytes and T cells, and some epithelial cells. CD21 is the receptor used by Epstein-Barr virus to infect B cells and is also the complement receptor for C3d. CD21 has also been shown to interact with a number of proteins, including CD23, CD19, annexin VI, CD81, iC3b, complement receptor 1 (CR1, CD35), and interferon-alpha 1 (IFN-α1).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
IHC-P - Verified
IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Björck P, et al. 1993. Eur. J. Immunol. 23:1771.
  2. Frémeaux-Bacchi V, et al. 1996. Eur. J. Immunol. 26:1497.
  3. Ling NR, et al. 1995. Clin. Exp. Immunol. 101:369.
  4. Wang, C, et al. 2011. BMC Immunol. 12:53. (IHC)
Product Citations
  1. Heesters BA, et al. 2021. J Exp Med. 218:. PubMed
  2. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
  3. Newell KL, et al. 2021. PLoS One. 16:e0244855. PubMed
  4. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  5. Stensland ZC, et al. 2022. iScience. 25:103626. PubMed
AB_11219188 (BioLegend Cat. No. 354902)

Antigen Details

Transmembrane protein, multiple SUSHI domains, approximate molecular weight 145 kD

Expressed on B cells, follicular dendritic cells, subsets of normal thymocytes and T cells, some epithelial cells

Epstein-Barr virus receptor on B cells used for infection
CD23, CD19, complement component 3, annexin VI, CD81, iC3b, complement receptor 1, interferon alpha 1
C3d, EBV
Cell Type
B cells, Dendritic cells, Epithelial cells, T cells, Thymocytes
Biology Area
Cell Biology, Complement, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Kishimoto T, Eds. 1997. Leukocyte Typing VI. Garland Publishing Inc.
2. Moore MD, et al. 1987. Proc. Natl. Acad. Sci. USA 84:9194.
3. Szakonyi G, et al. 2001. Science 292:1725.
4. Weis JJ, et al. 1984. Proc. Natl. Acad. Sci. USA 81:881.

Gene ID
1380 View all products for this Gene ID
View information about CD21 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 08.18.2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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