- HI111 (See other available formats)
- IV N231
- Other Names
- LFA-1α chain, Integrin αL subunit, αL Integrin, ITGAL
- Mouse IgG1, κ
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- Product Citations
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CD11a is a 170-180 kD type I transmembrane glycoprotein also known as LFA-1α chain and integrin αL subunit. CD11a non-covalently associates with integrin β2 (CD18) to form LFA-1. It is expressed on all leukocytes, including B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. It is absent on non-hematopoietic tissues and platelets. CD11a plays a central role in leukocyte cell-cell interactions and is important in lymphocyte costimulation. CD11a/CD18 binds to ICAM-1 (CD54), ICAM-2 (CD102), and ICAM-3 (CD50).Product Details
- Human, African Green, Baboon, Cattle (Bovine, Cow), Capuchin monkey, Chimpanzee, Cynomolgus, Dog (Canine), Horse (Equine), Rabbit (Lapine), Sheep (Ovine)
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IHC-F, WB, Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone HI111 epitope maps to the top region of the I domain that is close to the putative ligand-binding site surrounding the MIDAS (metal ion-dependent adhesion site). HI111 is specific for the closed confirmation of the integrin8. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, Western blotting2, and blocking of cell-cell interaction and inhibition the binding of ICAM-14. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301233 & 301234).
(PubMed link indicates BioLegend citation)
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press New York.
- Leite F, et al. 2002. Infec. Immun. 70:4336.
- Jiang Y, et al. 2005. Clin. Hemorheol. Microcircul. 32:261.
- BTchard D, et al. 2001. J. Immunol. 167:3099.
- Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200.
- Choi EY, et al. 2008. Blood 111:3607. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Ma Q, et al. 2002. J. Biol. Chem. 277:10638.
- Product Citations
AB_314140 (BioLegend Cat. No. 301202)
- Integrin, type I transmembrane glycoprotein, noncovalently linked with integrin β2 (CD18) forms LFA-1, 170-180 kD
- Adhesion, costimulation
- ICAM-1(CD54), ICAM-2(CD102), ICAM-3(CD50)
- Cell Type
- Leukocytes, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Lub M, et al. 1995. Immunol. Today 16:479.
2. Parsons J. 1996. Curr. Opin. Cell Biol. 8:146.
- Gene ID
- 3683 View all products for this Gene ID
- View information about CD11a on UniProt.org
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