Purified anti-human Basophils (2D7) Antibody

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Basophil/2D7 (See other available formats)
Mouse IgG1, κ
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Human peripheral blood mononuclear cells surface stained with CCR3 PE and then intracellularly stained with Basophil/2D7 Alexa Fluor®647
  • Basophilslash2D7_A647_122209
    Human peripheral blood mononuclear cells surface stained with CCR3 PE and then intracellularly stained with Basophil/2D7 Alexa Fluor®647
Cat # Size Price Quantity Avail. Save
346202 100 µg 156€
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The Basophil/2D7 mAb specifically recognizes the human basophil population. The antigen that this antibody binds is present in cell granules but its identity is still unknown.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
Lysates of purified basophils
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

ICFC - Quality tested
ICC, IHC-P, IHC-F - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications for the relevant formats include: immunofluorescence staining7, and immunohistochemistry of acetone-fixed frozen sections1 and formalin-fixed paraffin-embedded sections1-6.

Application References

(PubMed link indicates BioLegend citation)
  1. Kepley CL, et al. 1995. J. Immunol. 154:6548. (IHC)
  2. Irani AM, et al. 1998. J. Allergy Clin. Immunol. 1998 101:354. (IHC)
  3. Agis H, et al. 2006. J. Clin. Pathol. 59:396. (IHC)
  4. Plager DA, et al. 2006. J. Allergy Clin. Immunol. 117:626. (IHC)
  5. Sperr WR, et al. 2001. Blood 98:2200. (IHC)
  6. Aichberger KJ, et al. 2006. Blood 108:3538. (IHC)
  7. Kepley CL, et al. 1998. J. Allergy Clin. Immun. 102:304. (IF)
AB_1967135 (BioLegend Cat. No. 346202)

Antigen Details



Cell Type
Biology Area
Immunology, Innate Immunity
Gene ID
View information about Basophil on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 05.31.2013

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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