- Basophil/2D7 (See other available formats)
- Mouse IgG1, κ
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The Basophil/2D7 mAb specifically recognizes the human basophil population. The antigen that this antibody binds is present in cell granules but its identity is still unknown.Product Details
- Antibody Type
- Host Species
- Lysates of purified basophils
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ICFC - Quality tested
ICC, IHC-P, IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications for the relevant formats include: immunofluorescence staining7, and immunohistochemistry of acetone-fixed frozen sections1 and formalin-fixed paraffin-embedded sections1-6.
(PubMed link indicates BioLegend citation)
- Kepley CL, et al. 1995. J. Immunol. 154:6548. (IHC)
- Irani AM, et al. 1998. J. Allergy Clin. Immunol. 1998 101:354. (IHC)
- Agis H, et al. 2006. J. Clin. Pathol. 59:396. (IHC)
- Plager DA, et al. 2006. J. Allergy Clin. Immunol. 117:626. (IHC)
- Sperr WR, et al. 2001. Blood 98:2200. (IHC)
- Aichberger KJ, et al. 2006. Blood 108:3538. (IHC)
- Kepley CL, et al. 1998. J. Allergy Clin. Immun. 102:304. (IF)
AB_1967135 (BioLegend Cat. No. 346202)
- Cell Type
- Biology Area
- Immunology, Innate Immunity
- Gene ID
- View information about Basophil on UniProt.org
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