Purified anti-Hsp90α/β Antibody

Pricing & Availability
Clone
3H3C27 (See other available formats)
Other Names
Heat Shock Protein 90 kD Alpha (Cytosolic), Heat Shock 90 kD Protein 1, Beta, Class A Member 1, HSPC1, HSP86, HSP84, HSP89A, HSPCAL4, LAP2, EL52, HSP90B and HSP90N, HSP90, HSP90a
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
3H3C27_PURE_Hsp90alphaslashbeta_Antibody_1_WB_060916
Total cell lysate from 293T cells (lane 1), HeLa cells (lane 2), and NIH-3T3 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-HSP90 (clone 3H3C27) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
  • 3H3C27_PURE_Hsp90alphaslashbeta_Antibody_1_WB_060916
    Total cell lysate from 293T cells (lane 1), HeLa cells (lane 2), and NIH-3T3 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-HSP90 (clone 3H3C27) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
  • 3H3C27_PURE_Hsp90alphaslashbeta_Antibody_2_IP_060916
    Immunoprecipitation of HSP90 from Hela cell extracts. Lane 1 is 1% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-HSP90 antibody (clone 3H3C27, lane 4). Western blot was performed using anti-HSP90 antibody (clone 3H3C27).
Cat # Size Price Quantity Avail. Save
675402 100 µg 180€
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Description

Hsp90 is one of the most abundant molecular chaperones. It modulates the stability and transport of a diverse set of critical factors, which include regulatory, metabolic, organizational, and signaling proteins. There are two major isoforms: α and β. In humans, the Hsp90β isoform is constitutively expressed, whereas the Hsp90α isoform is expressed under stress conditions. The Hsp90 dimer acts in concert with other chaperones and is regulated by a number of co-chaperones/accessory proteins. The upregulation of Hsp90 is associated with tumor progression, the invasion and formation of metastases, and the development of drug resistance. Therefore, Hsp90 has emerged as an important target for cancer treatment.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human HSP90β recombinant protein (209-488 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IP - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 10-30 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Yang YS, et al. 2019. Nat Commun. 10:2958. PubMed
Publication Library
RRID
AB_2565512 (BioLegend Cat. No. 675402)

Antigen Details

Structure
Hsp90α: 732 amino acids with a predicted molecular weight of approximately 85 kD. Hsp90β: 724 amino acids with a predicted molecular weight of approximately 83 kD.
Distribution

Nucleus and cytoplasm.

Function
Hsp90 is one of the most abundant molecular chaperones. It modulates the stability and transport of critical factors that include regulatory, metabolic, organizational, and signaling proteins.
Interaction
NR3C1, FKBP4, FKBP5, PPID, PPP5C, STIP1, AHSA1, FNIP1, HSF1, SMYD3, TOM34, TERT, PTGES3, CHORDC1, DNAJC7, STUB1, UBE2N, PPP5C, PPP5C, p53/TP53, and CDK6.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance, Signal Transduction
Molecular Family
Heat Shock Proteins
Antigen References

1. Barrott JJ, et al. 2013. FEBS J. 280:1381.
2. Gorska M, et al. 2012. Front. Biosci. (Landmark Ed) 17:2269.
3. Barginear MF, et al. 2008. Curr. Cancer Drug Targets 8:522.
4. Arya R, et al. 2007. J. Biosci. 32:595.
5. Neckers L, et al. 2003. Curr. Opin. Oncol. 15:419.
6. Nemoto T, et al. 1997. J. Biol. Chem. 272:26179.

Gene ID
3320 View all products for this Gene ID 3326 View all products for this Gene ID
UniProt
View information about Hsp90alpha/beta on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 08.15.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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