Purified anti-Hsp70 Antibody (Previously Covance catalog# MMS-5103)

Pricing & Availability
Clone
4F8 (See other available formats)
Regulatory Status
RUO
Other Names
Heat shock 70kD protein 1A/1B, HSP70-1/HSP70-2, Heat shock-induced protein
Previously
Covance Catalog# MMS-5103
Isotype
Mouse IgG2b, κ/Mouse IgM, κ
Ave. Rating
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Product Citations
publications
4F8_Purified_Hsp70_Antibody_1_022519.png
Western blot of purified anti-Hsp70 antibody (clone 4F8). Lane 1: Molecular weight marker; Lane 2: 20 µg of HEK293 cell lysate. The blot was incubated with 1 µg/mL of the primary antibody for 60 minutes at room temperature, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 4F8_Purified_Hsp70_Antibody_1_022519.png
    Western blot of purified anti-Hsp70 antibody (clone 4F8). Lane 1: Molecular weight marker; Lane 2: 20 µg of HEK293 cell lysate. The blot was incubated with 1 µg/mL of the primary antibody for 60 minutes at room temperature, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 4F8_Purified_Hsp70_Antibody_2_022519.png
    IHC staining of purified anti-Hsp70 antibody (clone 4F8) on formalin-fixed paraffin-embedded human bladder tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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818101 100 µL 207€
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Description

Hsp70 is a member of the Hsp family of highly conserved proteins that play a role in a number of important functions. Their synthesis is increased upon environmental stress. They function by acting as molecular chaperones, facilitating protein refolding and preventing protein aggregation. It has been reported that extracellular Hsp70 also exhibits regulatory effects on innate and acquired immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against human recombinant HSP-70 protein over expressed in E. coli.
Formulation
Ammonium sulfate precipitated tissue culture supernatant.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1 - 10 µg per ml. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2564817 (BioLegend Cat. No. 818101)

Antigen Details

Structure
Expected MW: 70 kD
Biology Area
Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Heat Shock Proteins
Gene ID
3303 View all products for this Gene ID
UniProt
View information about Hsp70 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 04.27.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-Hsp70

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