Purified anti-Histone Lysine Demethylase (NO66) Antibody

Pricing & Availability
Clone
8A7-G10 (See other available formats)
Regulatory Status
RUO
Other Names
Chromosome 14 Open Reading Frame 169, ROX, hsNO66, NO66, Nucleolar Protein 66, Ribosomal Oxygenase NO66, MAPJD
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
8A7-G10_PURE_Histone_Lys_Demthylase_NO66_Antibody_WB_090915
70 µg of whole cell protein were separated by SDS-PAGE, transferred to a nitrocellulose membrane, and probed with αNO66 (clone 8A7-G10) diluted 1:100. The exposure time was 30 seconds.
  • 8A7-G10_PURE_Histone_Lys_Demthylase_NO66_Antibody_WB_090915
    70 µg of whole cell protein were separated by SDS-PAGE, transferred to a nitrocellulose membrane, and probed with αNO66 (clone 8A7-G10) diluted 1:100. The exposure time was 30 seconds.
Cat # Size Price Quantity Avail. Save
844902 100 µg 236,00€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Histone Lysine Demethylase (NO66) is an oxygenase that can act as both a histone lysine demethylase and a ribosomal histidine hydroxylase. It specfically demethylates 'Lys-4' (H3K4me) and 'Lys-36' (H3K36me) of histone H3, thereby playing a central role in the histone code.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein consisting of NO66 protein fragment (aa 423-427) and Hepatitis B virus capsid protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
RRID
AB_2566002 (BioLegend Cat. No. 844902)

Antigen Details

Structure
641 amino acids; 71 kD.
Distribution
Nucleus, nucleolus, and nucleoplasm.
Function
Preferentially demethylates trimethylated H3 'Lys-4' (H3K4me3) and momomethylated H3 'Lys-4' (H3K4me1) residues, while it has weaker activity for dimethylated H3 'Lys-36' (H3K36me2). Also catalyzes the hydroxylation of 60S ribosomal protein L8 on 'His-216' and acts as a regulator of osteoblast differentiation via its interaction with SP7/OSX by demethylating H3K4me and H3K36me, thereby inhibiting SP7/OSX-mediated promoter activation (by similarity). May also play a role in ribosome biogenesis and in the replication or remodeling of certain heterochromatic region. Participates in MYC-induced transcriptional activation.
Biology Area
Cell Biology, Neuroscience
Antigen References

1. Vasilatos S, et al. 2013. Carcinogenesis 34:1196.
2. Iwamori N, et al. 2011. Biol. Reprod. 84:1225.
3. Tao Y, et al. 2013. J. Biol. Chem. 288:16430.

Gene ID
79697 View all products for this Gene ID
UniProt
View information about NO66 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09.11.2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account