Loading...

Purified anti-GAPDH Antibody

Pricing & Availability
Clone
W17079A (See other available formats)
Regulatory Status
RUO
Other Names
glyceraldehyde-3-phosphate dehydrogenase
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
W17079A_PURE_GAPDH_Antibody_1_WB_042718_updated
Whole cell lysates (15 µg protein) from K562, PC3, HeLa, Molt-4, NTRA-2, NIH3T3, and UMR-106 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:10000 (0.05 µg/mL) Purified anti-GAPDH Antibody, clone W17079A. Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-rat-IgG secondary antibody (Cat. No. 405405, upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • W17079A_PURE_GAPDH_Antibody_1_WB_042718_updated
    Whole cell lysates (15 µg protein) from K562, PC3, HeLa, Molt-4, NTRA-2, NIH3T3, and UMR-106 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:10000 (0.05 µg/mL) Purified anti-GAPDH Antibody, clone W17079A. Proteins were visualized using chemiluminescence detection by incubating with 1:3000 dilution of HRP goat anti-rat-IgG secondary antibody (Cat. No. 405405, upper). 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control (lower). Lane M: MW ladder.
  • W17079A_PURE_GAPDH_Antibody_2_ICC_042418
    HeLa cells were fixed with cold methanol for 15 minutes and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with (A) 1:500 (1 µg/ml) purified rat IgG2a, κ isotype control antibody (Cat. No. 400502) or 1:500 (1 µg/ml, B), 1:2000 (0.25 µg/ml, C) and 1:5000 (0.1 µg/ml, D) anti-GAPDH antibody (clone W17079A) overnight at 4°C followed by Alexa Fluor® 488 (green) conjugated goat anti-rat IgG (Cat. No. 405418) for one hour at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective.
Compare all formats
Cat # Size Price Quantity Check Availability Save
607901 25 µg 81€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
607902 100 µg 203€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

GAPDH is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. GAPDH is a ubiquitously expressed and has a molecular mass of 36 kD. Though differentially expressed from tissue to tissue, GAPDH is frequently used as a loading control for assays involving mRNA and protein detection. In more recent studies, GAPDH has been shown to be involved in microtubule bundling, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer's diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant protein (161-335 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.0375-0.5 µg per ml (1:1000-1:16000 dilution). For immunocytochemistry, a concentration range of 0.25-1.0 μg/ml (1:500-1:2000 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone weakly reacts with rat GAPDH.

Product Citations
  1. Su T, et al. 2021. Metabolites. 11:. PubMed
  2. Hirschenberger M, et al. 2021. STAR Protoc. 2:100781. PubMed
  3. Hayn M, et al. 2021. Cell Reports. 35(7):109126. PubMed
  4. Nchioua R, et al. 2020. MBio. 11:00. PubMed
  5. Prelli Bozzo C, et al. 2021. Nat Commun. 12:4584. PubMed
  6. Srinivasachar Badarinarayan S, et al. 2020. Nucleic Acids Res. 48:10890. PubMed
  7. Bosso M, et al. 2021. Cell Rep. 36:109735. PubMed
  8. Bosso M, et al. 2021. STAR Protoc. 2:100236. PubMed
  9. Bosso M, et al. 2020. PLoS Pathog. 16:e1008752. PubMed
  10. Stanfield BA, et al. 2021. PLoS One. 16:e0245169. PubMed
  11. Nchioua R, et al. 2021. J Virol. 95:e0080821. PubMed
  12. Chamberlain N, et al. 2022. Int J Mol Sci. 23:. PubMed
  13. Chen HL, et al. 2021. Pharm Biol. 59:134. PubMed
  14. Simon Langer et al. 2019. eLife. 8 pii: e41930. PubMed
  15. Viereckl MJ, et al. 2022. Biomolecules. 12:. PubMed
  16. Hopfensperger K, et al. 2020. MBio. 11:00. PubMed
  17. Huq S, et al. 2020. Mol Cancer Ther. 19:1797. PubMed
  18. Hayashi M, et al. 2021. Viruses. 13:. PubMed
  19. Zhao Z, et al. 2021. PLoS Pathog. 17:e1009728. PubMed
RRID
AB_2734502 (BioLegend Cat. No. 607901)
AB_2734503 (BioLegend Cat. No. 607902)

Antigen Details

Structure
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Distribution

Ubiquitously expressed, locate in cytoplasm and perinuclear region.

Function
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Interaction
Interacts with TPPP. Interacts with EIF1AD and WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2.
Biology Area
Cell Biology, Western Blot Controls
Antigen References
  1. Ercolani L, et al. 1988. J. Biol. Chem. 263:15335.
  2. Meyer-Siegler K, et al. 1991. Proc. Natl. Acad. Sci. USA. 88:8460.
  3. Hara MR, Snyder SH. 2006. Cell. Mol. Neurobiol. 26:527.
  4. Zheng L, et al. 2003. Cell. 114:255.
  5. Wang Q, et al. 2005. FASEB J. 19:869.
  6. Bae BI, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:3405.
Gene ID
5590 View all products for this Gene ID
UniProt
View information about GAPDH on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04.27.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-GAPDH

  • Direct-Blot™ HRP anti-GAPDH

  • Alexa Fluor® 488 anti-GAPDH
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account