Purified anti-Filamin B Antibody

Pricing & Availability
Clone
W19323A (See other available formats)
Regulatory Status
RUO
Other Names
FLNB, ABP-280, ABP-278, TABP, TAP, Larsen Syndrome 1 (Autosomal Dominant) , Actin Binding Protein 278, Thyroid Autoantigen , Filamin Homolog 1, Filamin B, Beta ABP-280 Homolog, Beta-Filamin, Filamin-B, Filamin-3
Isotype
Rat IgG2a, λ
Ave. Rating
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Product Citations
publications
W19323A_PURE_FilaminB_Antibody_120220.png
Whole cell extracts (30 µg total protein) from the indicated cell lines were resolved by 3-8% Tris Acetate gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-Filamin B antibody (clone W19323A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W19323A_PURE_FilaminB_Antibody_120220.png
    Whole cell extracts (30 µg total protein) from the indicated cell lines were resolved by 3-8% Tris Acetate gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-Filamin B antibody (clone W19323A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
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942401 25 µg 80,00€
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942402 100 µg 200,00€
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Description

Filamin B is an actin-binding protein that functions as a scaffold protein linking the cytoskeleton to the plasma membrane. It is required for receptor-mediated signal transduction and is a key regulator of intracellular signaling through its cross-linking and anchoring of actin, which allows for communication between the cell membrane, transmembrane receptors, and the cytoskeletal network. As a scaffold protein,  it plays a vital role in membrane stability, cell migration, ion channel transport, mechano-transduction, and tissue morphogenesis. In endothelial cells, Filamin B is crucial to vascular development and endothelial cell motility and migration, which it accomplishes through its role as a scaffold between RAC-1 and VAV-2. Vital to skeletal development, cartilage development, and mineral absorption, FLNB (gene encoding Filamin B) mutations or deficiencies result in skeletal malformations, including joint dislocation, scoliosis, spondylocarpotarsal synostosis, Larsen syndrome, and atelosteogenesis. In cancers, Filamin B may regulate tumorigenesis and cancer cell apoptosis, transcription, and alternative splicing. Deficiencies in FLNB expression result in tumor growth and metastasis. 

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Synthetic human Filamin B peptide
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using HeLa cells fixed with 4% PFA and permeabilized with either methanol or Triton X-100. No staining was observed with either method.

This clone failed to adequately enrich Filamin B from HeLa whole cell extracts.

RRID
AB_2890863 (BioLegend Cat. No. 942401)
AB_2890863 (BioLegend Cat. No. 942402)

Antigen Details

Structure
Filamin B is a 2,602 amino acid protein with an expected molecular weight of 278 kD.
Distribution

Ubiquitously expressed/ Cytoskeleton

Function
Actin binding
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure
Molecular Family
Cytoskeletal Proteins
Antigen References

1. Baudier J, et al. 2018. J. Cell. Sci. 131:8.
2.Del Valle-Perez B, et al. 2009. J. Biol. Chem. 285:10748.
3. Lu J, et al. 2007. Hum. Mol. Genet. 16:1661.
4. Ma H, et al. 2020. Oncol. Rep. 43:1536

Gene ID
2317 View all products for this Gene ID
UniProt
View information about Filamin B on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12.02.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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