Purified anti-Dynamin-1 Antibody

Pricing & Availability
Clone
P83G4B6 (See other available formats)
Other Names
DNM1, DNM, DMN-1, dynamin 1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
P83G4B6_PURE_Dynamin1_Antibody_1_091417
Total lysates (15 µg protein) from Raji (negative control), A549 and mouse brain were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-Dynamin-1 antibody (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP goat anti-mouse-IgG secondary antibody for anti-Dynamin-1 antibody or HRP Donkey anti-rabbit IgG Antibody for GAPDH antibody. 1:2000 dilution of GAPDH (poly6314) antibody was used as a loading control (lower). Lane M: MW ladder.
  • P83G4B6_PURE_Dynamin1_Antibody_1_091417
    Total lysates (15 µg protein) from Raji (negative control), A549 and mouse brain were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-Dynamin-1 antibody (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP goat anti-mouse-IgG secondary antibody for anti-Dynamin-1 antibody or HRP Donkey anti-rabbit IgG Antibody for GAPDH antibody. 1:2000 dilution of GAPDH (poly6314) antibody was used as a loading control (lower). Lane M: MW ladder.
  • P83G4B6_PURE_Dynamin1_Antibody_2_091417
    Total lysates (15 µg protein) from human brain, mouse brain and rat brain were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:250 diluted (2 µg/mL) purified anti-Dynamin-1 antibody (upper). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP goat anti-mouse-IgG secondary antibody. 1:1000 (0.5 micro;g/mL) dilution of β-actin (2F1-1) antibody was used as a loading control (lower). Lane M: MW ladder.
  • P83G4B6_PURE_Dynamin1_Antibody_3_091417
    IHC staining of Formalin Fixed Paraffin Embedded (FFPE) normal human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with anti-Dynamin-1 antibody (Clone P83G4B6) at 5 µg/mL overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. Images were captured with a 40X objective.
  • P83G4B6_PURE_Dynamin1_Antibody_4_091417
    IHC staining of Formalin Fixed Paraffin Embedded (FFPE) mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with anti-Dynamin-1 antibody (Clone P83G4B6) at 5 µg/mL overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. Images were captured with a 40X objective.
  • P83G4B6_PURE_Dynamin1_Antibody_5_091417
    IHC staining of Formalin Fixed Paraffin Embedded (FFPE) rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with anti-Dynamin-1 antibody (Clone P83G4B6) at 5 µg/mL overnight at 4°C. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. Images were captured with a 40X objective.
Cat # Size Price Quantity Avail. Save
604001 25 µg 68€
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604002 100 µg 172€
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Description

Dynamin-1, also known as DMN-1, is a 100 kD protein with GTPase activity. Dynamin-1 is involved in several intracellular trafficking processes including synaptic vesicle recycling, neurotransmitter reuptake and receptor internalization.  In addition, Dynamin-1 may be necessary for the establishment and proper maintenance of mature neuronal structure. Studies have shown that Dynamin-1 is upregulated during new neurite formation. Conversely, Dynamin-1 is downregulated during neurite retraction. Silencing the initiation codon for Dynamin limits the formation of axon-like structures.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human Dynamin-1 (515-750a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1:250-1:2500 diluted (0.2 – 2 µg/mL). For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1:100-1:250 diluted (2 – 5 µg/mL). It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2716194 (BioLegend Cat. No. 604001)
AB_2716195 (BioLegend Cat. No. 604002)

Antigen Details

Structure
864 amino acids with a predicted molecular weight of 97 kD.
Distribution

Cytoplasm

Function
Involved in producing microtubule bundles and able to bind and hydrolyze GTP
Interaction
Interacts with CAV1, SH3GLB1, SH3GL1, SH3GL2, SH3GL3, PHOCN, PACSIN1, PACSIN2, PACSIN3, SNX9, MYO1E, SNX33, UNC119 and DIAPH1.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Obar RA, et al. 1990. Nature. 347:256.
  2. Chen-Hwang MC, et al. 2002. J Biol Chem. 277:17597.
  3. Grabs D, et al. 1997. J Biol Chem. 272:13419.
  4. Wunderlich L, et al. 1999. Cell. Signal. 11:25.
Gene ID
1759 View all products for this Gene ID
UniProt
View information about Dynamin-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09.15.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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