Purified anti-DEPTOR Antibody

Pricing & Availability
Clone
W17128A (See other available formats)
Other Names
DEPTOR, DEPDC6
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W17128A_Purified_DEPTOR_Antibody_052219_updated.png
Total cell lysates (15 µg total protein) from A431, NTERA2 (negative controls), Daudi, RPMI-8226 (positive controls) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL Purified anti-DEPTOR Antibody, clone W17128A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • W17128A_Purified_DEPTOR_Antibody_052219_updated.png
    Total cell lysates (15 µg total protein) from A431, NTERA2 (negative controls), Daudi, RPMI-8226 (positive controls) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 µg/mL Purified anti-DEPTOR Antibody, clone W17128A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
610201 25 µg 68€
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610202 100 µg 172€
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Description

DEPTOR/DEPDC6, found only in vertebrates, plays an important role in multiple cellular processes such as cell growth, autophagy, apoptosis, ER stress and adipogenesis. It inbibits mTORC1 and mTORC2 kinase activity through direct interaction with mTOR in these complexes. DEPTOR also associates with chromatin and may regulate transcription, and can act as either an oncogene or oncosuppressor depending on cell- or tissue-specific context.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, Hamster
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Human DEPTOR Protein (1-219 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783359 (BioLegend Cat. No. 610201)
AB_2783360 (BioLegend Cat. No. 610202)

Antigen Details

Distribution

Cytoplasm/ Nucleus, A little bit more in Cytoplasm

Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Chromatin Remodeling/Epigenetics, Signal Transduction, Transcription Factors
Molecular Family
Tumor Suppressors
Gene ID
64798 View all products for this Gene ID
UniProt
View information about DEPTOR on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05.22.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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