Purified anti-Cyclin D3 Antibody

Pricing & Availability
Clone
DCS-22 (See other available formats)
Regulatory Status
RUO
Other Names
G1/S-specific cyclin-D3, CCND3
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_DCS-22_PURE_CyclinD3_Antibody_WB_110117
Total lysates (15µg protein) from HeLa and HeLa Cyclin D3 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1µg/ml) purified anti- Cyclin D3 antibody, clone DCS-22 (upper). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody (cat#405306). Direct-Blot™ HRP anti-β-actin Antibody (Cat#643807) was used as a loading control (lower). Lane M is the Molecular weight ladder.
  • 1_DCS-22_PURE_CyclinD3_Antibody_WB_110117
    Total lysates (15µg protein) from HeLa and HeLa Cyclin D3 CRISPR/Cas9 knockout (KO) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1µg/ml) purified anti- Cyclin D3 antibody, clone DCS-22 (upper). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody (cat#405306). Direct-Blot™ HRP anti-β-actin Antibody (Cat#643807) was used as a loading control (lower). Lane M is the Molecular weight ladder.
  • 2_DCS-22_PURE_CyclinD3_Antibody_WB_112818
    Total cell lysates (15 µg total protein) from HEPG2 (low expressing control), HeLa, A549 and HEL cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1 µg/mL (1:500 dilution) of Purified anti-Cyclin D3 Antibody, clone DCS-22, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas.
  • 3_DCS-22_PURE_CyclinD3_Antibody_WB_031416
    Protein (15 µg) harvested from Jurkat, K562, Daudi and NIH3T3 were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal Cyclin D3 (clone DCS-22) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG (minimal x-reactivity) Antibody and chemiluminescence detection.
  • 4_DCS-22_PURE_CyclinD3_Antibody_ICFC_1_061418
    PHA-stimulated (3 days) human peripheral blood mononuclear cells were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with DAPI and purified anti-Cyclin D3 (clone DCS-22) antibody (right) or purified mouse IgG1, κ isotype control (left). Followed by anti-mouse IgG PE.
  • 5_DCS-22_PURE_CyclinD3_Antibody_ICFC_2_061418
    PHA-stimulated (3 days) human peripheral blood mononuclear cells were fixed and permeabilized with cold 70% ethanol, then intracellularly stained with DAPI and purified anti-Cyclin D3 (clone DCS-22) antibody (left) or purified mouse IgG1, κ isotype control (right). Followed by anti-mouse IgG PE.
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684902 100 µg 212€
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Description

D-type cyclins, including Cyclin D1, D2, and D3, are regulators of the Cyclin-dependent kinases 4 and 6 (Cdk4 and Cdk6) and mediate the growth factor-induced progression through the G1 phase in the cell cycle. Cyclins D1 and D3 are involved in cell-cycle progression in CDK-dependent manner and CDK-independent activation of transcription as well as metabolic control. CDKs promote cell cycle transitions in mammalian cells by phosphorylating key substrates cyclins. The active complex of cyclin D/CDK4 targets the retinoblastoma protein, which triggers the release of E2F transcription factors and activates G1/S-phase gene expression.

Abnormal expression of cyclins is involved in disease progression. Cyclin D3 is widely expressed in many tumor cells. Cyclin D3 gene is amplified in bladder carcinoma in situ. Genomic changes disrupting the expression of Cyclin D3 are associated with aberrant growth of several human B-lymphoid malignancies. Targeting Cyclin D3 by miR-138 could induce cell cycle arrest in hepatocellular carcinoma. Cyclin D3 was upregulated in breast cancer and could serve as predictor of breast cancer prognosis. Cyclin D3 can also be targeted in autoimmune diabetes and exerts a protective role on β cells by promoting their survival and fitness without affecting proliferation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length Cyclin D3 of human origin.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICFC, KO/KD-WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.5 µg per ml. For intracellular flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2572190 (BioLegend Cat. No. 684902)

Antigen Details

Structure
Major isoform has 291 amino acids and predicted molecular weight of 33 kD.
Distribution

Nucleus, cytoplasm and cell membrane.

Function
Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G1/S transition.
Interaction
CDK14, CDK4, CDK6, ATF5, EIF3K, CDKN1A, ICP0, POLR3GL, and TFIP11.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
Antigen References

1. Liu W, et al. 2004. Biochem. Biophys. Res. Commun. 321:954.
2. Sherr CJ. 1993. Cell 73:1059.
3. Saavedra-Ávila NA. 2014. Proc. Natl. Acad. Sci. USA 111:E3405.
4. Lukas J, et al. 1996. Mol. Cell Biol. 16:6917.
5. Wang W, et al. 2012. Carcinogenesis. 33:1113.
6. Cato MH, et al. 2011. Mol. Cell Biol. 31:127.
7. Zhang Q, et al. 2014. Mol. Cell Endocrinol. 382:583.
8. Chi Y, et al. 2015. Cancer Cell Int. 15:89.

Gene ID
896 View all products for this Gene ID
UniProt
View information about Cyclin D3 on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 11.01.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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