Purified anti-Complement C5b Antibody

Pricing & Availability
Clone
A18119A (See other available formats)
Regulatory Status
RUO
Other Names
complement C5 beta chain, C5a
Isotype
Mouse IgM, κ
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Product Citations
publications
A18119A_PURE_Complement-C5b_Antibody_1_120219
Western blot of purified anti-Complement C5b antibody (clone A18119A). Lane 1: Molecular weight marker; Lane 2: 10 µg of human plasma; Lane 3: 40 µg of HepG2 cell lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18119A_PURE_Complement-C5b_Antibody_1_120219
    Western blot of purified anti-Complement C5b antibody (clone A18119A). Lane 1: Molecular weight marker; Lane 2: 10 µg of human plasma; Lane 3: 40 µg of HepG2 cell lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18119A_PURE_Complement-C5b_Antibody_2_120219
    IHC staining of purified anti-Complement C5b antibody (clone A18119A) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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869901 25 µg 85€
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869902 100 µg 221€
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Description

The complement system is a part of eukaryotic innate defense system that enhances the clearance of exogenous microbes and damaged cells by antibodies and phagocytic cells. In vivo, the complement system is activated by three pathways: the classical complement pathway, the alternative complement pathway, and the lectin pathway. Complement C5 is a 1676 amino acid precursor protein synthesized in the liver. All three complement activation pathways culminate in the cleavage of C5, which produces two disulfide linked glycoproteins C5α and C5β. C5α is further cleaved by C5 convertase into C5a and C5α', the latter binds to C5β to form C5b, which in turn sequentially recruits C6-C9 to form the membrane attack complex (MAC). MAC destroys pathogens by forming a lytic pore in their membrane. Mutations affecting the Complement C5 gene lead to complement component 5 deficiency where patients demonstrate an increased propensity to develop severe recurrent infections.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH conjugated synthetic peptide corresponding to amino acids 428–454 of human complement C5 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 2.0 - 10 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody predominantly detects C5b in human cell line and human plasma. At high concentration (between 2 and 10 µg/mL), the full-length C5 is also detected at ~190 kD in human cell lines, but not in human plasma.

RRID
AB_2820182 (BioLegend Cat. No. 869901)
AB_2820183 (BioLegend Cat. No. 869902)

Antigen Details

Structure
C5b is a 655 amino acid protein with predicted and observed molecular mass of ~ 75 kD.
Distribution

Tissue Distribution: Predominantly in liver and plasma. Also expressed in the brain.
Cellular Distribution: Plasma membrane and extracellular

Function
Complement C5b generation and assembly of MAC facilitates the lysis and destruction of pathogens.
Interaction
Interacts with C5a chain
Ligand/Receptor
Staphylococus aureus protein SSL5, binds C6 (transiently)
Cell Type
Astrocytes, Mature Neurons, Microglia
Biology Area
Cell Biology, Complement, Neurodegeneration, Neuroinflammation, Neuroscience
Molecular Family
Innate Immune Signaling
Antigen References
  1. Vlaicu SI, et al. 2019. Front Immunol. 10:1054
  2. Fishelson Z, et al. 2019. Front Immunol. 10:752
  3. Bergseth G, et al. 2013. Mol Immunol. 56(3):232
Gene ID
727 View all products for this Gene ID
UniProt
View information about Complement C5b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12.02.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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  • Purified anti-Complement C5b

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