- TD.1 (See other available formats)
- Regulatory Status
- Other Names
- Clathrin heavy chain 1, clathrin, heavy polypeptide-like 2, clathrin heavy chain on chromosome 17
Covance Catalog# MMS-427P
- Mouse IgG1, κ
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- Product Citations
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Clathrin consists of three heavy chains (192 kD) and three light chains (LCa or LCb, 25-29 kD) and self assembles into a polyhedral cytoskeletal element. The self assembly facilitates clathrin's central role in membrane sorting and selective transport within the cell.Product Details
- Human, Drosophila
- Antibody Type
- Host Species
- This monoclonal antibody was raised using the globular N-terminal domain of clathrin heavy chain.
- Phosphate-buffered solution + 0.03% Thimerosal.
- The antibody was purified by affinity chromatography.
- 1 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
WB - Quality tested
ICC - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody reacts with clathrin heavy chains (CHC). The antibody is useful for immunoblotting of clathrin heavy chains from cow CHC and immunofluorescence. It does not react with native clathrin in immunoprecipitation.
Reactivity to Drosophila was only verified with the purified format.
- Additional Product Notes
For Western blotting, the suggested use of this reagent is 5 -10 µg/ml in human, mouse, and rat and 1-10 µg/ml in Drosophila.
(PubMed link indicates BioLegend citation)
- Hood FE and Royle SJ. 2009. J Cell Sci. 122:2185-2190. (ICC) PubMed
- Jiang R, et al. 2000. J Biol Chem 275(12):8439-8447. (IP)
- Product Citations
AB_2564779 (BioLegend Cat. No. 813901)
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
- Gene ID
- 1213 View all products for this Gene ID
- View information about Clathrin Heavy Chain on UniProt.org
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