- W17190B (See other available formats)
- Regulatory Status
- Other Names
- CHIP, STIP1 Homology And U-Box Containing Protein 1, Carboxy Terminus Of Hsp70-Interacting Protein, RING-Type E3 Ubiquitin Transferase CHIP, E3 Ubiquitin-Protein Ligase CHIP, CLL-Associated Antigen KW-8, Antigen NY-CO-7, Heat Shock Protein A Binding
- Rat IgG2b, κ
- Ave. Rating
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- Product Citations
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CHIP is an E3 ubiquitin-protein ligase known to ubiquitinate misfolded chaperone-bound substrates and target them for proteasomal degradation. Hsp70, Hsc70, and Hsp90 are three chaperone proteins with which CHIP interacts. CHIP mediates polyubiquitination of DNA polymerase beta at 'Lys-41', 'Lys-61' and 'Lys-81', thereby plays a role in base-excision repair. CHIP can also negatively regulate the suppressive function of regulatory T-cells during inflammation by mediating the ubiquitination and degradation of FOXP3 in a HSPA1A/B-dependent manner. CHIP also negatively regulates TGF-beta signaling by modulating the basal levels of SMAD3 via ubiquitin-mediated degradation. Mutations in CHIP cause spinocerebellar ataxia type 16.Product Details
- Human, Mouse, Rat
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
IHC-P - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
AB_2810779 (BioLegend Cat. No. 865701)
AB_2810780 (BioLegend Cat. No. 865702)
- CHIP is a 303 amino acid protein with an apparent molecular mass of ~ 35 kD.
Tissue Distribution: Brain, heart, skeletal muscle, kidney, liver, and spleen
Cellular Distribution: Cytosol, nucleus, and endoplasmic reticulum
- CHIP is involved in protein ubiquitination, protein degradation, and neurodegeneration.
- Ubiquitin, ATXN3, EPHA2, SMAD3, UBE4B, VCP/p97.
- Cell Type
- Glutamatergic Neurons, Mature Neurons, Neurons, Tregs
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neurodegeneration, Neuroscience, Signal Transduction, Ubiquitin/Protein Degradation
- Molecular Family
- Enzymes and Regulators, Heat Shock Proteins, Postsynaptic proteins, Phospho-Proteins, Protein Modifiers
- Antigen References
- Zhan S, et al. 2017. Int Rev Immunol. 36(5):300.
- Cao Z, et al. 2016. Oncotarget. 7(20):29864.
- Gene ID
- 10273 View all products for this Gene ID 56424 View all products for this Gene ID 287155 View all products for this Gene ID
- View information about CHIP on UniProt.org
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