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Purified anti-β-Amyloid, 1-16 Antibody (Previously Covance catalog# SIG-39322)

Pricing & Availability
Clone
Poly8029 (See other available formats)
Regulatory Status
RUO
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Signet Catalog# 9322-005
Covance Catalog# SIG-39322
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly8029_Pure_B-Amyloid_Antibody_110817
IHC staining of anti-β-Amyloid, 1-16 antibody (clone Poly8029) on formalin-fixed paraffin-embedded Human Alzheimer's disease brain tissue. Following antigen retrieval using 70% formic acid, the tissue was incubated with 1 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm.
  • Poly8029_Pure_B-Amyloid_Antibody_110817
    IHC staining of anti-β-Amyloid, 1-16 antibody (clone Poly8029) on formalin-fixed paraffin-embedded Human Alzheimer's disease brain tissue. Following antigen retrieval using 70% formic acid, the tissue was incubated with 1 µg/mL of the primary antibody for 60 minutes at room temperature. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm.
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802901 50 µg 405€
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Description

Amyloid beta (Aβ or Amyloid beta) denotes peptides of 36-43 amino acids in length that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. The peptides result from the amyloid precursor protein (APP), which is cut by certain enzymes to yield Aβ. Aβ molecules can aggregate to form oligomers (known as "seeds") which are believed to be able to induce other Aβ molecules to also take the misfolded oligomeric form, leading to a chain reaction akin to a prion infection. The seeds or the resulting amyloid plaques are toxic to nerve cells. The other protein implicated in Alzheimer's disease, tau protein, also forms such prion-like misfolded oligomers, and there is some evidence that misfolded Aβ can induce tau to misfold.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Polyclonal
Host Species
Rabbit
Formulation
Phosphate-buffered solution (no preservatives or carrier proteins).
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested
ELISA, WB - Reported in the literature, not verified in house 
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1-5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting, immunohistochemistry (IHC), and ELISA.

 

This antibody reacts with all isoforms of beta amyloid. It was developed using beta amyloid peptide (amino acids 1-16) conjugated to KLH.

 

This product may contain other non-IgG subtypes.

 

Pre-treatment: For optimal staining, tissue sections should be pre-treated with an antigen unmasking solution such as 70% Formic acid.

 

Positive control: Human Alzheimer’s disease brain tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Robertson RT, et al. 2009. Neuroscience 3:1334-46. (IHC) PubMed
  2. Chen KF, et al. 2014. Dis Model Mech. 4:445 (IHC) PubMed
Product Citations
  1. Younan ND, et al. 2018. J Biol Chem. 293:13090. PubMed
  2. Chen K, et al. 2014. Dis Model Mech. 7:445-458. PubMed
  3. Robertson R, et al. 2009. Neuroscience. 164:1334-1346. PubMed
  4. Condello C, et al. 2018. Proc Natl Acad Sci U S A. 115:E782. PubMed
RRID
AB_2564699 (BioLegend Cat. No. 802901)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid, 1-16 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 02.09.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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