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Purified anti-Artemis Antibody

Pricing & Availability
Clone
13A9C90 (See other available formats)
Regulatory Status
RUO
Other Names
Protein artemis, DNA cross-link repair 1C protein (DCLRE1C), ASCID, SCIDA, SNM1 homolog C (SNM1C)
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_13A9C90_PURE_Artemis_Antibody_113018
Total cell lysates (15 µg total protein) from PC-3, A549 and HaCat cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Artemis Antibody, clones 15E7C90 and 13A9C90, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Cell lysates were loaded in order of increasing DCLRE1C mRNA expression levels (source: Human Protein Atlas).
  • 1_13A9C90_PURE_Artemis_Antibody_113018
    Total cell lysates (15 µg total protein) from PC-3, A549 and HaCat cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-Artemis Antibody, clones 15E7C90 and 13A9C90, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Cell lysates were loaded in order of increasing DCLRE1C mRNA expression levels (source: Human Protein Atlas).
  • 2_13A9C90_PURE_Artemis_Antibody_122216
    Jurkat and A20 cell lysates (12 µg each) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-Artemis (clone 13A9C90, 1 µg/ml) antibody. Proteins were visualized using an HRP goat anti-mouse IgG antibody (clone Poly4053) and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
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692902 100 µg 203€
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Description

Artemis, also known as DCLRE1C, is a nuclear protein belonging to the metallo-β-lactamase superfamily. Artemis has been found to be required for the assembling of B cell antibody genes and T cell receptor genes from individual V, D, and J segments. During V(D)J recombination, Artemis forms a complex with DNA-dependent protein kinase (DNA-‘PK) and cuts the DNA with hairpin structure. Deficiency in Artemis results in blocking of V(D)J recombination and failure to produce mature B and T cells, which is known as the condition of severe combined immune deficiency (SCID). Artemis is also found to be involved in the termini processing step of double-strand break (DSB) repair. Artemis-deficient cells are more sensitive to DSB induced by ionizing radiation or chemical agents such as bleomycin and neocarzinostatin.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human anti-Artemis recombinant protein (347-692 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 13A9C90 recognizes both human and mouse Artemis; Clone 15E7C90 only recognizes human Artemis

RRID
AB_2632771 (BioLegend Cat. No. 692902)

Antigen Details

Structure
692 amino acids with a predicted molecular weight of 78.4 kD. Four isoforms produced by alternative splicing.
Distribution

Nucleus.

Function
Artemis is a nuclear protein exhibiting single-strand-specific 5'-3' exonuclease activity and endonuclease activity. It plays crucial roles in V(D)J recombination and DNA repair.
Interaction
Interacts with ATM, BRCA1, PRKDC and TP53BP1.
Cell Type
Mesenchymal Stem Cells
Biology Area
Cell Biology, DNA Repair/Replication, Stem Cells
Antigen References

1. Chang HH, Lieber MR. 2016. Nucleic Acids Res. 44:4991.
2. Volk T, et al. 2015. Hum Mol Genet. 24:7361.
3. Waide EH, et al. 2015. J Immunol. 195:3171.
4. Lee PP, et al. 2013. Clin Immunol. 149:464.
5. Köcher S, et al. 2012. Nucleic Acids Res. 40:8336.
6. Beucher A, et al. 2009. EMBO J. 28:3413.

Gene ID
64421 View all products for this Gene ID
UniProt
View information about Artemis on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12.22.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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