- Poly18231 (See other available formats)
- Regulatory Status
- Other Names
- Gamma-secretase subunit APH-1A, aph-1alpha, presenilin-stabilization factor, CGI-78, anterior pharynx defective 1 homolog A, presenilin-stabilization factor
Covance Catalog# PRB-550P
- Rabbit Polyclonal IgG
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- Product Citations
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The gamma secretase is a multiprotein complex consisting of presenilin, nicastrin, Aph-1 and Pen-2; all four proteins are necessary for full proteolytic activity. The gamma secretase complex catalyzes cleavage of a number of integral membrane proteins including Amyloid precursor protein, Notch, Erb4 and E-cadherin. While Aph-1 stabilizes the presenilin holoprotein within the gamma secretase complex, Pen-2 is required for endoproteolysis of presenilin and activation of the gamma secretase. The Aph-1 homologue on human chromosome 1 is termed Aph-1a and on human chromosome 15 is referred to as Aph-1b.Product Details
- Antibody Type
- Host Species
- The antibody Aph-1a, 245-265 (Poly18231) was raised against the human Aph-1aL/C-terminal region and recognizes the sequence CRRQEDSRVMVYSALRIPPED of human Aph-1aL.
- Phosphate-buffered solution, pH 7.2.
- The antibody was purified by affinity chromatography.
- 1.0 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting.
The optimal working dilution should be determined for each specific assay condition.
• WB: 1:1,000*
- Application Notes
This antibody is effective in immunoblotting (WB).
*Expected MW: 29 kD
WB Positive Control: Human Brain lysate
BioLegend also offers Poly18232 (823201) which recognizes residues 92-115 of the Aph-1aL/ S Loop.
This product may contain other non-IgG subtypes.
(PubMed link indicates BioLegend citation)
- Gu Y, Chen F, Sanjo N, Kawarai T, Hasegawa H, Duthie M, Li W, Ruan X, Luthra A, Mount HT, Tandon A, Fraser PE, St George-Hyslop P. Aph-1 interacts with mature and immature forms of presenilins and nicastrin and may play a role in maturation of the presenilin.nicastrin complexes. J Biol Chem 278:7374-7380, 2003.
- Product Citations
AB_2564862 (BioLegend Cat. No. 823101)
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- Gene ID
- 51107 View all products for this Gene ID
- View information about Aph-1a, 245-265 on UniProt.org
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