Postsynaptic Antibody Sampler Kit

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Posynaptic_Antibody_Sampler_Kit_1_111518
Western blot of purified anti-Pan-Shank antibody (clone N23B/49). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 5 µg/mL of N23B/49 or mouse IgG1, κ overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • Posynaptic_Antibody_Sampler_Kit_1_111518
    Western blot of purified anti-Pan-Shank antibody (clone N23B/49). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 5 µg/mL of N23B/49 or mouse IgG1, κ overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • Posynaptic_Antibody_Sampler_Kit_2_111518
    IHC staining of purified anti-Pan-Shank antibody (clone N23B/49) on formalin-fixed paraffin-embedded rat cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Posynaptic_Antibody_Sampler_Kit_3_111518
    IHC staining of purified anti-MAGUK (pan reactive) antibody (clone K28/86) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin HRP kit (Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Posynaptic_Antibody_Sampler_Kit_5_111518
    Western blot of purified anti-PSD-93 (Chapsyn-110) antibody (clone N18/28). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • Posynaptic_Antibody_Sampler_Kit_6_111518
    Western blot of purified anti-SAP102 antibody (clone N19/2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of mouse brain membrane lysate; Lane 4: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
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899911 1 kit 228€
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Description

The Postsynaptic Antibody Sampler Kit offers flexibility for sampling and detection of major targets localized to the postsynaptic area. These antibodies are highly suitable for WB and IHC applications.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
Specificity Format Clone Size Reactivity Isotype
Anti-Pan-Shank Purified N23B/49 25 μg Human, Mouse, Rat Mouse IgG1, κ
Anti-MAGUK (pan reactive) Purified K28/86 25 μl Human, Mouse, Rat Mouse IgG1
Anti-PSD95 Purified  K28/74 25 μl Human, Mouse, Rat Mouse IgG1
Anti-PSD-93 (Chapsyn-110) Purified N18/28 25 μl Human, Mouse, Rat Mouse IgG2a
Anti-SAP102 Purified N19/2 25 μl Human, Mouse, Rat Mouse IgG1

* For detailed information about each specificity, please refer to the datasheets of the individual products. 

Product Details

Formulation
Please refer to individual product datasheets of the purified formats for details.
Preparation
All antibodies in this kit were purified by affinity chromatography.
Storage & Handling
Upon receipt, store undiluted at 2-8°C.
Application

WB, IHC-P - Quality tested

Recommended Usage

Each lot of antibodies in this kit is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows:

Anti-Pan-Shank: 1.0 - 5.0 µg/ml
Anti-PSD95: 0.1 - 5.0 µg/ml
Anti-PSD-93 (Chapsyn-110): 1.0 µg/ml
Anti-SAP102: 10 µg/ml

For immunohistochemistry on formalin-fixed paraffin-embedded tissue, the suggested use of this reagent is as follows:

Anti-MAGUK (pan reactive): 0.5 - 1.0 µg/ml

It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For validated or reported applications for these antibodies, please see individual product datasheets.

Related FAQs

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Go To Top Version: 1    Revision Date: 11.16.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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