- 10C4B40; A15158B;
- Other Names
- Signal transducer and activator of transcription 1 (STAT1), Transcription factor ISGF-3 components p91/p84
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- Product Citations
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STAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcription factor. Phosphorylation at serine 727 is required for full transcriptional activator function. Two isoforms of STAT1, with apparent molecular weights of 88 (STAT1α) and 91 (STAT1β) kD, exist as a result of alternative RNA processing. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.Product Details
- Kit Contents
Product Application Clone Size Reactivity Isotype Purified anti-STAT1 Antibody WB, IP 10C4B40 25 μg Human, Mouse Mouse IgG1, κ Purified STAT1 Phospho (Ser727) Antibody WB, ICC, ICFC A15158B 25 μg Human, Mouse Mouse IgG1, κ
* For detailed information about each specificity, please refer to the datasheets of the individual products.
- Human, Mouse
- Please refer to individual product datasheets for immunogen information
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality testeed
- Recommended Usage
Each lot of antibodies in this set is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows:
Clone 10C4B40: 1.0 µg/mL
Clone A15158B: 0.5 - 2.5 µg/mL
It is recommended that the reagent be titrated for optimal performance for each application.
For recommended usage information for additional applications, please refer to the datasheet of the individual products.
- Application Notes
The serine 727 phosphorylation site is only found in STAT1α; it is absent in STAT1β. Therefore, clone A15158B only detects a single band corresponding to the serine 727-phosphorylated α isoform, while clone 10C4B40 detects both α and β isoforms.
- Two isoforms of STAT1 exist as a result of alternative splicing. Isoform α is a 750 amino acid protein with a predicted molecular weight of 87 kD; the β isoform is a 712 amino acid protein with a predicted molecular weight of 83 kD.
Ubiquitously expressed/Nucleus and cytosol
- Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to the nucleus to act as a transcription factor. Mediates responses to type I (IFN-α/β) and II interferon (IFN-γ), EGF, PDGF, and IL-6.
- Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.
- Cell Type
- Mesenchymal cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction, Stem Cells, Transcription Factors
- Molecular Family
- Nuclear Markers, Phospho-Proteins
- Antigen References
- Durbin JE, et al. 1996. Cell. 84:443-50.
- Darnell JE Jr, et al. 1994. Science. 264:1415-21.
- Chen X, et al. 1998. Cell. 93:827-39.
- Ramana CV, et al. 2000. Oncogene. 19:2619-27.
- Gene ID
- 6772 View all products for this Gene ID
- View information about STAT1 on UniProt.org
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