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PerCP/Cyanine5.5 anti-mouse TCR Vγ2 Antibody

Pricing & Availability
Clone
UC3-10A6 (See other available formats)
Regulatory Status
RUO
Other Names
Vγ2 T-cell receptor, T cell receptor gamma 2, TCR Vγ4 (Tonegawa nomenclature)
Isotype
Armenian Hamster IgG
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Product Citations
publications
UC3-10A6_PERCP_CYANINE5-5_TCRVgamma2_Antibody_090619
C57BL/6 splenocytes were stained with CD3 FITC and TCR V?2 (clone UC3-10A6) PerCP/Cyanine5.5 (left) or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (right).
  • UC3-10A6_PERCP_CYANINE5-5_TCRVgamma2_Antibody_090619
    C57BL/6 splenocytes were stained with CD3 FITC and TCR V?2 (clone UC3-10A6) PerCP/Cyanine5.5 (left) or Armenian Hamster IgG PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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137711 25 µg 109€
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137712 100 µg 287€
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Description

T cell receptor (TCR) Vγ2 bearing T lymphocytes make up a significant proportion of γδ TCR cells in late fetal and adult peripheral lymphoid tissues. TCR γδ T cells may play a role in immunological surveillance for stress-induced self-antigens. The frequency of Vγ2 expression in different strains varies from 12% to 54% in the TCR γδ repertoire. Variations in the levels of Vγ2+ cells are not associated with MHC haplotype.

Clone UC3-10A6 reacts with the Vγ2 segment as designated by the Garman nomenclature.1 Mouse TCR Vγ2 is also known as TCR Vγ4 under the Heilig & Tonegawa nomenclature system.

Several TCR γ and δ chain nomenclature systems exist. In order to consolidate the various nomenclature systems, we have created charts for corresponding names across some of the most popular naming methods. Click here to see charts.
 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
G8 mouse T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Dent AL, et al. 1990. Nature 343:714.
  2. Kelly KA, et al. 1993. Int. Immunol. 5:331.
  3. Sperling AI, et al. 1992. J. Immunol. 149:3200.
  4. Sperling AI, et al. 1997. J. Immunol. 159:86.
RRID
AB_2814039 (BioLegend Cat. No. 137711)
AB_2814040 (BioLegend Cat. No. 137712)

Antigen Details

Structure
A member of Ig superfamily, associated with TCR δ chain and CD3 complex.
Distribution

T cell subset in fetal and adult peripheral lymphoid tissues and thymus.

Function
Might play a role in a previously unrecognized form of immunological surveillance for stress-induced self-antigens.
Ligand/Receptor
Antigens
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Allison JP, et al. 1991. Annu. Rev. Immunol. 9:679.
2. O’Brien RL, et al. 2000. J. Immunol. 165:6472.

Gene ID
110067 View all products for this Gene ID
UniProt
View information about TCR Vgamma2 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 09.06.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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