PerCP/Cyanine5.5 anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
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Product Citations
publications
RA3-6B2_PerCPCyanine55_CD45R-B220_Antibody_091919.png
C57BL/6 mouse splenocytes stained with CD3 APC and CD45R/B220 (clone RA3-6B2) PerCP/Cyanine5.5 (left) or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right)
  • RA3-6B2_PerCPCyanine55_CD45R-B220_Antibody_091919.png
    C57BL/6 mouse splenocytes stained with CD3 APC and CD45R/B220 (clone RA3-6B2) PerCP/Cyanine5.5 (left) or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (right)
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
103235 25 µg 60€
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103236 100 µg 164€
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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Cat (Feline)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, and immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6.

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Product Citations
  1. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  2. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  3. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  4. Echevarría-Vargas IM, et al. 2018. EMBO Mol Med. 10:e8446. PubMed
  5. Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed
  6. Beloor J et al. 2018. Cell host & microbe. 23(4):549-556 . PubMed
  7. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  8. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  9. Di Liberto G et al. 2018. Cell. 175(2):458-471 . PubMed
  10. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  11. Melissa M Remy et al. 2017. Cell host & microbe. 22(3):354-365 . PubMed
  12. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  13. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  14. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  15. Page N, et al. 2018. Immunity. 48:937. PubMed
  16. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  17. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  18. Hilpert C, et al. 2019. J Immunol. 203:3068. PubMed
  19. Olson WJ, et al. 2019. Cell Rep. 28:2878. PubMed
  20. Leal AS, et al. 2019. Sci Rep. 5.286111111. PubMed
  21. van der Poel CE, et al. 2019. Cell Rep. 29:2745. PubMed
  22. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  23. Lutzmann M, et al. 2019. Cell Rep. 28:2851. PubMed
  24. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  25. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  26. Lang J, et al. 2020. Nat Commun. 11:1338. PubMed
  27. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  28. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  29. Kobayashi T, et al. 2019. Cell. 176:982. PubMed
  30. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
  31. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  32. Elsner RA, et al. 2020. Cell Reports. 29(9):2796-2809.e5.. PubMed
  33. Rivadeneira DB, et al. 2020. Immunity. 51(3):548-560. PubMed
  34. Baker GJ, et al. 2020. Cell Syst. 0.647222222. PubMed
  35. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  36. Pereira J, et al. 2010. PLoS One. 5:e9277. PubMed
  37. Sasaki I, et al. 2012. Blood. 120:4733. PubMed
  38. Liu B, et al. 2013. Development. 140:780. PubMed
  39. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
  40. Kaku H, et al. 2014. J Immunol. 193:5904. PubMed
  41. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  42. Minamitani T, et al. 2015. Proc Natl Acad Sci U S A. 112: 11612-11617. PubMed
  43. Kong S, et al. 2015. J Immunol. 195: 3685 - 3693. PubMed
  44. JI B, et al. 2016. Cell Death Differ. 23:759-75. PubMed
  45. Macal M, et al. 2016. J Immunol. 196: 1900 - 1909. PubMed
  46. Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed
  47. Yasuoka T, et al. 2016. PLoS One. 11: 0157395. PubMed
  48. Qi T, et al. 2020. Cell Rep. 33:108452. PubMed
  49. Simoni L, et al. 2020. Cell Rep. 33:108330. PubMed
  50. Yang J, et al. 2020. Nature. 586:572. PubMed
  51. Montel-Hagen A, et al. 2020. Cell Rep. 33:108320. PubMed
  52. Chen L, et al. 2020. J Exp Med. 217:00:00. PubMed
  53. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  54. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  55. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  56. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  57. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  58. Giampazolias E, et al. 2021. Cell. . PubMed
RRID
AB_893356 (BioLegend Cat. No. 103235)
AB_893354 (BioLegend Cat. No. 103236)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 09.19.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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