PerCP/Cyanine5.5 anti-mouse CD38 Antibody

Pricing & Availability
Clone
90 (See other available formats)
Other Names
T10
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
90_PerCPCyanine5dot5_CD38_Antibody_020819.png
C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CD38 (clone 90) PerCP/Cyanine5.5 (left) or Rat IgG2a, ? PerCP/Cyanine5.5 isotype control (right).
  • 90_PerCPCyanine5dot5_CD38_Antibody_020819.png
    C57BL/6 mouse splenocytes were stained with CD45R/B220 FITC and CD38 (clone 90) PerCP/Cyanine5.5 (left) or Rat IgG2a, ? PerCP/Cyanine5.5 isotype control (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
102721 25 µg 116€
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102722 100 µg 248€
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Description

CD38 is a 42 kD glycoprotein, also known as T10. It is an ADP-ribosyl hydrolase, expressed on B cells, NK cells, a subset of T cells, brain, muscle, and kidney. In mouse, CD38 expression is downregulated on germinal center B cells and plasma cells, whereas this is not the case for humans. By functioning as both a cyclase and a hydrolase, CD38 mediates lymphocyte activation, as well as adhesion and metabolism of cADPR and NAADP. CD31 is the ligand of CD38.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow pre-B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry1,2 of acetone-fixed frozen sections, and induction of B cell proliferation1.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Oliver AM, et al. 1997. J. Immunol. 158:1108.
  2. Howard M, et al. 1993. Science 262:1056.
Product Citations
  1. Krueger CC, et al. 2019. Front Immunol. 10:1831. PubMed
  2. Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
  3. Kräutler NJ, et al. 2020. Cell Reports. 30(4):997-1012.e6.. PubMed
  4. Muri J, et al. 2020. Cell Reports. 29(9):2731-2744.e4.. PubMed
RRID
AB_2563332 (BioLegend Cat. No. 102721)
AB_2563333 (BioLegend Cat. No. 102722)

Antigen Details

Structure
ADP-ribosyl hydrolase, 42 kD
Distribution

B cells, NK cells, T cell subset, brain, muscle, kidney

Function
Regulation of cellular activation/proliferation, adhesion, metabolism of cADPR and NAADP
Ligand/Receptor
CD31, hyaluronic acid
Cell Type
B cells, NK cells, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Shubinsky G, et al. 1997. Immunity 7:315.
3. Cesano A, et al. 1998. J. Immunol. 160:1106.
4. Cockayne DA, et al. 1998. Blood 92:1324.

Gene ID
12494 View all products for this Gene ID
UniProt
View information about CD38 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 02.08.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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