- HM-CCR5 (See other available formats)
- Other Names
- CCR5, C-C chemokine receptor type 5, HIV-1 fusion co-receptor
- Armenian Hamster IgG
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- Product Citations
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CD195 is a 45 kD chemokine receptor also known as CCR5. CD195 is a seven transmembrane-spanning G protein-associated molecule expressed on macrophages, a T cell subset, and in the heart, liver, and spleen. CD195 regulates lymphocyte chemotaxis and transendothelial migration during inflammatory processes. CD195 interacts with several ligands including RANTES, MCP-1, MIP-1α, and MIP-1β.Product Details
- Antibody Type
- Host Species
- Armenian Hamster
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
CCR5 is expressed at low density on activated cells. For successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 107006) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 107004) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
AB_2616985 (BioLegend Cat. No. 107015)
AB_2616986 (BioLegend Cat. No. 107016)
- β-chemokine receptor, 45 kD
- Macrophages, T cell subset, heart, spleen, liver
- Lymphocyte chemotaxis and transendothelial migration during inflammation, signaling through seven transmembrane-spanning G proteins
- RANTES, MCP-1, MIP-1α, and MIP-1β
- Cell Type
- Macrophages, T cells, Dendritic cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Napolitano M, et al. 1990. J. Exp. Med. 172:285.
3. Meyer A, et al. 1996. J. Biol. Chem. 271:14445.
4. Boring, et al. 1996. J. Biol. Chem. 271:7551.
- Gene ID
- 12774 View all products for this Gene ID
- View information about CD195 on UniProt.org
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
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