C57BL/6 mouse splenocytes were stained with B220 APC and IgD (clone 11-26c.2a) PE/Dazzle™ 594 (top) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (bottom).
C57BL/6 mouse splenocytes were stained with B220 APC and IgD (clone 11-26c.2a) PE/Dazzle™ 594 (top) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (bottom).
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.
Excitation Laser
Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes
The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3.
Application References
(PubMed link indicates BioLegend citation)
Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
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