PE/Dazzle™ 594 anti-mouse/human Helios Antibody

Pricing & Availability
Clone
22F6 (See other available formats)
Regulatory Status
RUO
Other Names
IKZF2, IKAROS family zinc finger 2, ANF1A2, ZNF1A2, ZNFN1A2
Isotype
Armenian Hamster IgG
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Product Citations
publications
22F6_PEDZL_Helios_Antibody_ICFC_1_060115
C57BL/6 splenocytes were surface stained with CD4 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with Helios (clone 22F6) PE/Dazzle™ 594.
  • 22F6_PEDZL_Helios_Antibody_ICFC_1_060115
    C57BL/6 splenocytes were surface stained with CD4 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with Helios (clone 22F6) PE/Dazzle™ 594.
  • 22F6_PEDZL_Helios_Antibody_ICFC_2_060115
    Human peripheral blood lymphocytes were surface stained with CD4 Alexa Fluor® 647 and then treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with Helios (clone 22F6) PE/Dazzle™ 594.
See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Check Availability Save
137231 25 tests 132,00€
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137232 100 tests 308,00€
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Description

Helios is a member of the Ikaros family of zinc finger transcription factors.  It contains a C-terminal region composed of 2 zinc-finger domains that mediate dimerization between the family members.  Helios was originally cloned from a mouse thymoma line. It is mainly expressed in peripheral T cells and thymocytes.  It is found at high levels in a subpopulation of regulatory T cells.  Helios plays an important role in T cell development and homeostasis.  Overexpression of Helios profoundly alters αβ T cell differentiation and activation.  It has been determined that silencing of Helios in B cells is critical for maintaining normal B cell function.  Helios is also involved in tumor immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Helios peptide (aa 51-107)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the True-Nuclear™ 10X Perm Buffer (Cat. No. 424403).

View more applications data for a different format of this clone in our Scientific Poster Library.

Application References

(PubMed link indicates BioLegend citation)
  1. Thornton AM, et al. 2010. J. Immunol. 184:1. PubMed
  2. Verhagen J and Wraith D. 2010. J. Immunol. 185:7129.
  3. Stone B, et al. 2012. Clin Immunol. 145:153. PubMed
  4. Vaeth M, et al. 2012. PNAS. 109:16258. PubMed
  5. Angin M, et al. 2014. PLoS One. 9:86920. PubMed
  6. Bedke T, et al. 2014. Immunol Cell Biol. PubMed
  7. Liu Y, et al. 2014. Am J Physiol Gastrointest Liver Physiol. 307:177. PubMed
  8. Verhagen J and Wraith DC. 2014. J. Immunol. Methods. S0022. (FC) PubMed
Product Citations
  1. Yilmaz B, et al. 2021. Cell Host Microbe. 29(4):650-663.e9. PubMed
  2. Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed
RRID
AB_2565796 (BioLegend Cat. No. 137231)
AB_2565797 (BioLegend Cat. No. 137232)

Antigen Details

Structure
A zinc finger nuclear transcription factor of the Ikaros family, containing 2 zinc-finger domains in C-terminal region.
Distribution

Regulatory T cells (Tregs), conventional T cells, thymocytes.

Function
Plays an important role in T cell development and homeostasis, involved in tumor immunity.
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Kelly CM, et al.  1998. Curr. Biol. 8:508.
2. Dovat S, et al. 2005. J. Immunol. 175:3508.
3. Cortes M, et al. 1999. Curr. Opin. Immunol. 11:167.
4. Cai Q, et al. 2009. J. Immunol. 183:2303.
5. Zhang Z, et al. 2007. Blood 109:2190.
6. Hahm K, et al. 1998. Genes Dev. 12:782.

Gene ID
22779 View all products for this Gene ID 22807 View all products for this Gene ID
UniProt
View information about Helios on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06.08.2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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