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PE/Cyanine7 anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Regulatory Status
RUO
Other Names
B220
Isotype
Rat IgG2a, κ
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Product Citations
publications
RA3-6B2_PECy7_090507
C57BL/6 mouse splenocytes stained with RA3-6B2 PE/Cyanine7
  • RA3-6B2_PECy7_090507
    C57BL/6 mouse splenocytes stained with RA3-6B2 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
103221 25 µg 70€
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103222 100 µg 159€
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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6, and spatial biology (IBEX)14,15.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  14. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  15. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  2. Nagamachi A, et al. 2021. J Clin Invest. 131:. PubMed
  3. Aït-Azzouzene D, et al. 2010. J Immunol. 185:1015. PubMed
  4. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  5. Sakamoto A, et al. 2017. Int Immunol. . 10.1093/intimm/dxx054. PubMed
  6. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  7. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  8. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
  9. Ouchida R, et al. 2012. Proc Natl Acad Sci U S A. 109:e2699. PubMed
  10. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  11. Ersching J et al. 2017. Immunity. 46(6):1045-1058 . PubMed
  12. Manion KP, et al. 2020. PLoS One. 15:e0236664. PubMed
  13. Gutiérrez-Gutiérrez Ó, et al. 2021. EMBO Rep. 22:e52905. PubMed
  14. Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
  15. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  16. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  17. Pérez‐Mazliah D et al. 2017. EBioMedicine. 24:216-230 . PubMed
  18. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  19. Suzuki M, et al. 2021. Sci Adv. 7:. PubMed
  20. Ohyagi M, et al. 2021. Nat Commun. 12:7344. PubMed
  21. Majumder P, et al. 2021. J Immunol. 206:2221. PubMed
  22. Mathew NR, et al. 2021. Cell Reports. 35(12):109286. PubMed
  23. Sharma PK, et al. 2018. Cancer Gene Ther. 25:27. PubMed
  24. Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed
  25. Li Y, et al. 2022. Clin Transl Immunology. 11:e1362. PubMed
  26. Lee RD, et al. 2021. Nat Commun. 12:6843. PubMed
  27. Heizmann B, et al. 2013. J Exp Med. 210:2823. PubMed
  28. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  29. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  30. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  31. Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
  32. Li X, et al. 2020. Mol Ther. 28:2430. PubMed
  33. Stacey M, et al. 2011. J Immunol. 187:2944. PubMed
  34. Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
  35. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  36. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
  37. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
  38. Kobayashi M, et al. 2020. Bio Protoc. 10:e3601. PubMed
  39. Fennell LM, et al. 2020. EMBO J. 39:e103303. PubMed
  40. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  41. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  42. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  43. Zhu H, et al. 2019. Nat Commun. 10:1084. PubMed
  44. Tran NT, et al. 2020. STAR Protocols. 1(1):100028. PubMed
  45. Albarrán-Juárez J, et al. 2016. Atherosclerosis. 251:445-53. PubMed
  46. Wanner-Seleznik GM, et al. 2020. Immunohorizons. 0.644444444. PubMed
  47. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  48. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  49. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  50. Crouse B, et al. 2020. NPJ Vaccines. 0.277083333. PubMed
  51. Rozanski C, et al. 2015. J Immunol. 194:4717. PubMed
  52. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  53. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  54. Chen RJ, et al. 2022. iScience. 25:105595. PubMed
  55. Timilshina M, et al. 2017. PLoS One. 10.1371/journal.pone.0168942. PubMed
  56. Lamoureux J, et al. 2007. J Exp Med. 204:2853. PubMed
  57. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  58. Kiyohara H, et al. 2018. Cell Mol Gastroenterol Hepatol. 7:135. PubMed
  59. Ha TC, et al. 2020. Hum Gene Ther. 32:458. PubMed
  60. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  61. Wang W, et al. 2021. J Am Heart Assoc. 10:e019142. PubMed
  62. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  63. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  64. Tozuka M, et al. 2016. J Dermatol Sci. 82: 38-45. PubMed
  65. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  66. Lu X, et al. 2019. Cell Rep. 28:472. PubMed
  67. Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed
  68. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  69. Agrawal M, et al. 2022. Blood. 140:1094. PubMed
  70. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  71. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  72. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  73. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  74. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  75. Ulrich V, et al. 2016. EMBO Mol Med. 8: 643 - 653. PubMed
  76. Valkenburg S, et al. 2016. Sci Rep. 6:22666. PubMed
  77. Xie X, et al. 2015. Sci Rep. 5: 18115. PubMed
  78. El-Zaatari M, et al. 2017. Gastroenterology. 10.1053/j.gastro.2017.09.002. PubMed
  79. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  80. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  81. Read BJ, et al. 2022. Cell Rep. 38:110217. PubMed
  82. Zhong C, et al. 2021. J Virol. 95:e0092521. PubMed
  83. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  84. Tomita T, et al. 2021. Nat Commun. 12:3655. PubMed
  85. Matsushita N, et al. 2016. Sci Rep. 6:31266. PubMed
  86. Timilshina M, et al. 2020. Cell Reports. 27(10):2948-2961.e7.. PubMed
  87. Rappe JCF, et al. 2021. J Exp Med. 218:. PubMed
  88. Zhang M, et al. 2022. iScience. 25:104490. PubMed
  89. Rodrigues KA, et al. 2021. Sci Adv. 7:eabj6538. PubMed
  90. Liu A, et al. 2022. Front Cell Infect Microbiol. 12:960208. PubMed
  91. Wirasinha RC, et al. 2021. J Exp Med. 218: . PubMed
  92. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  93. Nabekura T, et al. 2020. Immunity. 96:52. PubMed
RRID
AB_313004 (BioLegend Cat. No. 103221)
AB_313005 (BioLegend Cat. No. 103222)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD45R/B220 Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F,FC,3D IHC
APC anti-mouse/human CD45R/B220 RA3-6B2 FC
Biotin anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
FITC anti-mouse/human CD45R/B220 RA3-6B2 FC
PE anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Cyanine5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
PE/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Cyanine7 anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 488 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC
Alexa Fluor® 647 anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Alexa Fluor® 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP anti-mouse/human CD45R/B220 RA3-6B2 FC
PerCP/Cyanine5.5 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 421™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F
Brilliant Violet 570™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 650™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 605™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 785™ anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 510™ anti-mouse/human CD45R/B220 RA3-6B2 FC,IHC-F,SB
Purified anti-mouse/human CD45R/B220 (Maxpar® Ready) RA3-6B2 FC,CyTOF®
Brilliant Violet 711™ anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Dazzle™ 594 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 750 anti-mouse/human CD45R/B220 RA3-6B2 FC
Brilliant Violet 750™ anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-A0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Spark Blue™ 550 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark NIR™ 685 anti-mouse/human CD45R/B220 RA3-6B2 FC
TotalSeq™-B0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
Ultra-LEAF™ Purified anti-mouse/human CD45R/B220 RA3-6B2 FC,CyTOF®,IHC-F,IHC-P,IP,Activ
TotalSeq™-C0103 anti-mouse/human CD45R/B220 RA3-6B2 PG
PE/Fire™ 640 anti-mouse/human CD45R/B220 RA3-6B2 FC
APC/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
PE/Fire™ 700 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Violet™ 538 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 581 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark YG™ 570 anti-mouse/human CD45R/B220 RA3-6B2 IHC-F
PE/Fire™ 810 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark Blue™ 574 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Violet™ 423 anti-mouse/human CD45R/B220 Antibody RA3-6B2 FC
Spark Red™ 718 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark UV™ 387 anti-mouse/human CD45R/B220 RA3-6B2 FC
Spark PLUS UV™ 395 anti-mouse/human CD45R/B220 RA3-6B2 FC
Go To Top Version: 2    Revision Date: 04.25.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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